Difference between revisions of "PutP"

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|style="background:#ABCDEF;" align="center"|'''Function''' || proline uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || proline uptake
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03220 putP]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03220 putP]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 8.619   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 8.619   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[putC]]'', ''[[putR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[putC]]'', ''[[putR]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12116&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03220 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03220 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03220 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ycgO_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ycgO_context.gif]]

Revision as of 12:06, 13 May 2013

  • Description: high affinity proline permease

Gene name putP
Synonyms ycgO
Essential no
Product high affinity proline permease
Function proline uptake
Gene expression levels in SubtiExpress: putP
MW, pI 48 kDa, 8.619
Gene length, protein length 1347 bp, 449 aa
Immediate neighbours putC, putR
Sequences Protein DNA Advanced_DNA
Genetic context
YcgO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PutP expression.png
























Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids membrane proteins

This gene is a member of the following regulons

PutR regulon

The gene

Basic information

  • Locus tag: BSU03220

Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: uptake of proline coupled to the uptake of sodium ions
  • Protein family: sodium solute symporter family (SSS; TC2A.21)
  • Paralogous protein(s): OpuE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of proline (PutR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Boris R Belitsky
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
J Mol Biol: 2011, 413(2);321-36
[PubMed:21840319] [WorldCat.org] [DOI] (I p)