Program

Wednesday 10th April 2013

▪ 8:00 – 9.00 REGISTRATION ▪ 9:00 – 9:10 – Welcome Introduction

Session I Metabolism

CHAIR: Leendert Hamoen

• 9:10 – 9:25 – Ken-ichi Yoshida, Kobe University, Japan
  • Bacillus subtilis possesses two distinct triosephosphate isomerases
• 9:30 – 9:45 – Kambiz Morabbi Heravi, University of Stuttgart, DE
  • Mannitol utilization system of Bacillus subtilis: activation of the mtl promoters by MtlR
• 9:50 – 10:05 – Marian Wenzel, University of Stuttgart,DE
  • Development of a self-inducible Bacillus subtilis expression system for application in high-cell-density fermentations.
• 10:10 – 10:25 – Elisabeth Härtig, Technical University Braunschweig, DE
  • Transcriptional activation by the AlsR regulator of Bacillus subtilis comprises effector binding, oligomerization, DNA binding and DNA bending
• 10:30 – 10:50 – COFFEE BREAK

Session II Biofilms

CHAIR: Dirk-Jan Scheffers

• 10:50 – 11:05 – Laura Hobley, University of Dundee, UK
  • BslA is a novel self-polymerising protein that forms a hydrophobic layer around Bacillus subtilis biofilms
• 11:10 – 11:25 – Akos T Kovacs, University of Jena, DE
  • Living on the surface: Biofilm formation and surface motility of Bacillus subtilis
• 11:30 – 11:45 – Ole Økstad, University of Oslo, NO
  • Cyclic diguanylate (c-di-GMP) regulation of biofilm formation in Bacillus subtilis
• 11:50 – 12:05 – Jörg Stülke, University of Göttingen, DE
  • c-di-AMP, an essential signalling nucleotide in Bacillus subtilis
• 12:10 – 12:25 – Lynne Cairns, University of Dundee, UK
  • Investigation of the role of the motility protein, YvyG, and potential regulation by tyrosine phosphorylation in Bacillus subtilis
• 12:30 – 13:40 LUNCH (+ BACIP MEETING)

Session III: Beyond Bacillus subtilis, a focus on non-subtilis species

CHAIR: Jan Maarten van Dijl

• 13:40 – 13:55 – Anne-Brit Kolstø, University of Oslo, NO
  • TBA
• 14:00 – 14:15 – Rebecca Biedendieck, Technische Universitat, Braunschweig, DE
  • Production and secretion of recombinant proteins in Bacillus megaterium
• 14:20 – 14:35 – Per Bullough, University of Sheffield, UK
  • Surface architecture of endospores of the Bacillus cereus/anthracis/thuringiensis family at the subnanometer scale.
• 14:40 – 14:55 – Susanne Pohl, Newcastle University, UK
  • Oxidative stress in Bacillus anthracis
• 15:00 – 15:15 –Mette Dines-Cantor, Chr. Hansen A/S, Hørsholm, DK
  • Screening for new Bacillus-based probiotic feed additives for the pig industry.
• 15:20 – 15:35 – Jurgen Seppen, Amsterdam Medical Centre, NL
  • The utility Bacillus subtilis for intestinal gene transfer.
• 15:40 – 16:00 – COFFEE BREAK

Session IV: Spores/sporulation

CHAIR: Nicola Stanley-Wall

• 16:00 – 16:15 – Stanley Brul, University of Amsterdam, NL
  • Functional genomics for food preservation: inhibiting bacterial spore germination
• 16:20 – 16:35 – Ewa Bukowska-Faniband, University of Lund, SE
  • Cortex synthesis during Bacillus subtilis sporulation depends on the transpeptidase activity of SpoVD
• 16:40 – 16:55 – Robyn EIJLANDER, University of Groningen, NL
  • SporeWeb: Interactive visualization of gene regulatory networks during the complete sporulation cycle of Bacillus subtilis
• 17:00 – 17:15– Katie Jameson, University of York, UK
  • Regulating DnaA to maintain diploidy at the onset of sporulation
• 17:20 – 17:35 – Katja Nagler, German Aerospace Centre, Cologne, DE
  • High salinity has negative effects on Bacillus subtilis spore germination and outgrowth
• 17.40 – 17.55– Patrik Florek, Slovak Academy of Sciences, SK
  • Characterization of Bacillus three-component SpoIISABC toxin-antitoxin system
• 18.00 – 19.30 BACELL STEERING GROUP MEETING

Thursday April 11th 2013

Session V: Synthetic Biology

CHAIR: Thomas Wiegert

• 9:00 – 9:15 – Elbrich Hendricks, University of Groningen, NL
  • iGEM team Groningen 2012: Food Wardens – Grand Prize Winners
• 9:20 – 9:35 – Francois Coutte, University of Lille, FR
  • Genetic engineering of Bacillus subtilis assisted by computational modelling to overproduce lipopeptide biosurfactants
• 9:40 – 9:55 – Maarten Mols, University of Groningen, NL
  • Improving the Bacilllus cell factory by cell envelope engineering
• 10:00 – 10:15 – Tjeerd van Ris, DSM, Delft, NL
  • Improved enzyme production by codon pair optimization
• 10:20 – 10:35 – Alexander Webb, Imperial College London, UK
  • Parasight
• 10:40 – 11:00 – COFFEE BREAK

Session VI: Cell walls and secretion

CHAIR: Ole Økstad

• 11:00 – 11:15 – Patricia Dominguez-Cuevas, Newcastle University, UK
  • Actin cytoskeleton Mbl homologue coordinates cell wall hydrolases and LytE activity during cell elongation.
• 11:20 – 11:35 – David Noone, Trinity College Dublin, IE
  • Several regulatory mechanisms combine to make expression and cell association of the CwlO D,L-endopeptidase-type autolysin responsive to growth conditions in Bacillus subtilis
• 11:40 – 11:55 – Dirk-Jan Scheffers, University of Groningen, NL
  • Substrate availability guides peptidoglycan synthesis
• 12:00 – 12:15 – Thomas Wiegert, University of Applied Sciences Zittau, DE
  • Influence of the intramembrane cleaving protease RasP on protein secretion in Bacillus subtilis
• 12:20 – 12:35 – Vivianne Goosens, University Medical Centre Groningen, NL
  • A role for TatAC in Bacillus subtilis twin-arginine protein translocation
• 12:40 – 13:40 – LUNCH

Session VII: Regulation & Replication

CHAIR: Henrik Strahl

• 13:40 – 13:55 – Sara Kesel, Ludwig-Maximilians-University, DE
  • Immediate and heterogeneous response of the LiaF-LiaS-LiaR two-component system of Bacillus subtilis to the peptide antibiotic bacitracin
• 14:00 – 14:15 –Jakob Engman, Lund University, SE
  • Aggregation of the YjbH adaptor protein as a mechanism of Spx regulation.
• 14:20 – 14:35 – Fabian Commichau, University of Göttingen, DE
  • Essential genes in Bacillus subtilis: a re-evaluation after 10 years
• 14:40 – 14:55– Thorsten Mascher, Ludwig-Maximilians-University, DE
  • (Tools for studying the) Regulatory dynamics of peptide antibiotic detoxification modules in Bacillus subtilis
• 15:00 – 15:15 – Vladimir Bidnenko, INRA, Jouy en Josas, FR
  • Distinct activities of co-essential Bacillus subtilis flap-endonucleases, Pol I and YpcP, in DNA replication and repair
• 15:20 – 15:35 – Panos Soultanas, University of Nottingham, UK
  • Functional interplay of DnaE polymerase, DnaG primase and DnaC helicase within a ternary complex during lagging strand DNA replication in Bacillus subtilis
• 15:40 – 16:00 – COFFEE BREAK

Session VIII: Division

CHAIR: Alan Nielson

• 16:00 – 16:15 – Peter Graumann, Universitat Freiburg, DE
  • Movement of up to 3.4mm long MreB filaments at various angles underneath the cell membrane and their intrinsic membrane affinity
• 16:20 – 16:35 – Hiraku Takada, Tokyo University of Agriculture, Japan
  • Involvement of essential lipid synthetic enzyme, PlsX, in the cell division machinery
• 16:40 – 16:55 – Ilkay Celik, Newcastle University, UK
  • Bacillus minimal divisome
• 17:00 – 17:15 – Robert Cleverley, Newcastle University, UK
  • Structure of the major divisome protein EzrA
• 17:20 – 17:35 – Alex Dajkovic, University of California Irvine, USA
  • Organization of FtsZ polymers in living Bacillus subtilis cells
• 17:40 – 17:55 – Romain Mercier, Newcastle University, UK
  • Excess membrane synthesis drives a primitive mode of cell proliferation