Program

Wednesday 10th April 2013

▪ 8:00 – 9.00 REGISTRATION ▪ 9:00 – 9:10 – Welcome Introduction

Session I Metabolism

CHAIR: Leendert Hamoen

• 9:10 – 9:25 – Ken-ichi Yoshida, Kobe University, Japan
  • Bacillus subtilis possesses two distinct triosephosphate isomerases
• 9:30 – 9:45 – Kambiz Morabbi Heravi, University of Stuttgart, DE
  • Mannitol utilization system of Bacillus subtilis: activation of the mtl promoters by MtlR
• 9:50 – 10:05 – Marian Wenzel, University of Stuttgart,DE
  • Development of a self-inducible Bacillus subtilis expression system for application in high-cell-density fermentations.
• 10:10 – 10:25 – Elisabeth Härtig, Technical University Braunschweig, DE
  • Transcriptional activation by the AlsR regulator of Bacillus subtilis comprises effector binding, oligomerization, DNA binding and DNA bending
• 10:30 – 10:50 – COFFEE BREAK

Session II Biofilms

CHAIR: Dirkjan Scheffers

• 10:50 – 11:05 – Laura Hobley, University of Dundee, UK
  • BslA is a novel self-polymerising protein that forms a hydrophobic layer around Bacillus subtilis biofilms
• 11:10 – 11:25 – Akos Kovacs, University of Jena, DE
  • Living on the surface: Biofilm formation and surface motility of Bacillus subtilis
• 11:30 – 11:45 – Ole Okstad, University of Oslo, NO
  • Cyclic diguanylate (c-di-GMP) regulation of biofilm formation in Bacillus subtilis
• 11:50 – 12:05 – Jörg Stülke, University of Göttingen, DE
  • c-di-AMP, an essential signalling nucleotide in Bacillus subtilis
• 12:10 – 12:25 – Lynne Cairns, University of Dundee, UK
  • Investigation of the role of the motility protein, YvyG, and potential regulation by tyrosine phosphorylation in Bacillus subtilis
• 12:30 – 13:40 LUNCH (+ BACIP MEETING)

Session III: Beyond Bacillus subtilis, a focus on non-subtilis species CHAIR: Jan Maarten van Dijl 13:40 – 13:55 – Anne-Brit Kolstø, University of Oslo, NO ▪ TBA 14:00 – 14:15 – Rebecca Biedendieck, Technische Universitat, Braunschweig, DE ▪ Production and secretion of recombinant proteins in Bacillus megaterium 14:20 – 14:35 – Per Bullough, University of Sheffield, UK ▪ Surface architecture of endospores of the Bacillus cereus/anthracis/thuringiensis family at the subnanometer scale. 14:40 – 14:55 – Susanne Pohl, Newcastle University, UK ▪ Oxidative stress in Bacillus anthracis 15:00 – 15:15 –Mette Dines-Cantor, Chr. Hansen A/S, Hørsholm, DK ▪ Screening for new Bacillus-based probiotic feed additives for the pig industry. 15:20 – 15:35 – Jurgen Seppen, Amsterdam Medical Centre, NL ▪ The utility Bacillus subtilis for intestinal gene transfer. 15:40 – 16:00 – COFFEE BREAK

Session IV: Spores/sporulation CHAIR: Nicola Stanley-Wall 16:00 – 16:15 – Stanley Brul, University of Amsterdam, NL ▪ Functional genomics for food preservation: inhibiting bacterial spore germination 16:20 – 16:35 – Ewa Bukowska-Faniband, University of Lund, SE ▪ Cortex synthesis during Bacillus subtilis sporulation depends on the transpeptidase activity of SpoVD 16:40 – 16:55 – Robyn EIJLANDER, University of Groningen, NL ▪ SporeWeb: Interactive visualization of gene regulatory networks during the complete sporulation cycle of Bacillus subtilis 17:00 – 17:15– Katie Jameson, University of York, UK ▪ Regulating DnaA to maintain diploidy at the onset of sporulation 17:20 – 17:35 – Katja Nagler, German Aerospace Centre, Cologne, DE ▪ High salinity has negative effects on Bacillus subtilis spore germination and outgrowth 17.40 – 17.55– Patrik Florek, Slovak Academy of Sciences, SK ▪ Characterization of Bacillus three-component SpoIISABC toxin-antitoxin system 18.00 – 19.30 BACELL STEERING GROUP MEETING

19:30 – WINE RECEPTION AND DINNER AT THE Great North Museum, Barras Bridge

Thursday April 11th 2013 Session V: Synthetic Biology CHAIR: Thomas Wiegert 9:00 – 9:15 – Elbrich Hendricks, University of Groningen, NL ▪ iGEM team Groningen 2012: Food Wardens – Grand Prize Winners 9:20 – 9:35 – Francois Coutte, University of Lille, FR ▪ Genetic engineering of Bacillus subtilis assisted by computational modelling to overproduce lipopeptide biosurfactants 9:40 – 9:55 – Maarten Mols, University of Groningen, NL ▪ Improving the Bacilllus cell factory by cell envelope engineering 10:00 – 10:15 – Tjeerd van Ris, DSM, Delft, NL ▪ Improved enzyme production by codon pair optimization 10:20 – 10:35 – Alexander Webb, Imperial College London, UK ▪ Parasight 10:40 – 11:00 – COFFEE BREAK

Session VI: Cell walls and secretion CHAIR: Ole Okstad 11:00 – 11:15 – Patricia Dominguez-Cuevas, Newcastle University, UK ▪ Actin cytoskeleton Mbi homologue coordinates cell wall hydrolases and LytE activity during cell elongation. 11:20 – 11:35 – David Noone, Trinity College Dublin, IE ▪ Several regulatory mechanisms combine to make expression and cell association of the CwIO D,L-endopeptidase-type autolysin responsive to growth conditions in Bacillus subtilis 11:40 – 11:55 – Dirk-Jan Scheffers, University of Groningen, NL ▪ Substrate availability guides peptidoglycan synthesis 12:00 – 12:15 – Thomas Wiegert, University of Applied Sciences Zittau, DE ▪ Influence of the intramembrane cleaving protease RasP on protein secretion in Bacillus subtilis 12:20 – 12:35 – Vivianne Goosens, University Medical Centre Groningen, NL ▪ A role for TatAC in Bacillus subtilis twin-arginine protein translocation 12:40 – 13:40 – LUNCH

Session VII: Regulation & Replication CHAIR: Henrik Strahl 13:40 – 13:55 – Sara Kesel, Ludwig-Maximilians-University, DE ▪ Immediate and heterogeneous response of the LiaFSR two-component system of Bacillus subtilis to the peptide antibiotic bacitracin 14:00 – 14:15 –Jakob Engman, Lund University, SE • Aggregation of the YjbH adaptor protein as a mechanism of Spx regulation. 14:20 – 14:35 –Fabian Commichau, University of Gottingen, DE ▪ Essential genes in Bacillus subtilis: a re-evaluation after 10 years 14:40 – 14:55– Thorsten Mascher, Ludwig-Maximilians-University, DE ▪ (Tools for studying the) Regulatory dynamics of peptide antibiotic detoxification modules in Bacillus subtilis 15:00 – 15:15 – Vladimir Bidnenko, INRA, Jouy en Josas, FR ▪ Distinct activities of co-essential Bacillus subtilis flap-endonucleases, Pol I and YpcP, in DNA replication and repair 15:20 – 15:35 – Panos Soultanas, University of Nottingham, UK ▪ Functional interplay of DnaE polymerase, DnaG primase and DnaC helicase within a ternary complex during lagging strand DNA replication in Bacillus subtilis 15:40 – 16:00 – COFFEE BREAK

Session VIII: Division CHAIR: Alan Nielson 16:00 – 16:15 – Peter Graumann, Universitat Freiburg, DE ▪ Movement of up to 3.4mm long MreB filaments at various angles underneath the cell membrane and their intrinsic membrane affinity 16:20 – 16:35 – Hiraku Takada, Tokyo University of Agriculture, Japan ▪ Involvement of essential lipid synthetic enzyme, PlsX, in the cell division machinery 16:40 – 16:55 – Ilkay Celik, Newcastle University, UK ▪ Bacillus minimal divisome 17:00 – 17:15 – Robert Cleverley, Newcastle University, UK ▪ Structure of the major divisome protein EzrA 17:20 – 17:35 – Alex Dajkovic, University of California Irvine, USA ▪ Organization of FtsZ polymers in living Bacillus subtilis cells 17:40 – 17:55 – Romain Mercier, Newcastle University, UK ▪ Excess membrane synthesis drives a primitive mode of cell proliferation

END OF MEETING

Tuesday April 24th 2012 – Morning

• 8:00-8:45 - REGISTRATION
• 9:00-9:10 – Welcome Introduction Kevin Devine

Session I: Modeling and Networks

CHAIR: Sierd Bron

• 9:10-9:30 – BACELL Systems – Past, Present, Future
  • Jan Maarten van Dijl (University Medical Center Groningen)

• 9:30-9:50 – Unraveling the Small Regulatory RNA Network of Bacillus subtilis
  • Ruben Mars (University Medical Center Groningen)

• 9:50-10:10 – A Computational Stressosome Model Consolidates Hypotheses about Environmental Activation of SigB the General Stress response
  • Ulf Liebal (University of Rostock)

• 10:10-10:30 – Reconciling Molecular Regulatory Mechanisms with Noise Patterns of Bacterial Metabolic Promoters in Induced and Repressed States
  • Matthieu Jules (Institut Micalis, INRA)

• 10:30 – 11:00 – COFFEE BREAK

Session II: Cell Wall

CHAIR: Colin Harwood

• 11:00-11:20 – The WalR/WalK, PhoP/PhoR and SigI/RsgI Regulatory Systems of Bacillus subtilis Cooperate to Control Cell Wall Metabolism during Growth and Under Stress Conditions
  • Letal Salzberg (Trinity College Dublin)

• 11:20-11:40 – Adaptation of Bacillus subtilis to High Salinity: Osmotic Induction of the Cell Wall Hydrolase YqiI
  • Kathleen Fischer (Philipps-Universität Marburg)

• 11:40-12:00 – Regulatory and Cellular Dynamics of the Lia-Response of Bacillus subtilis
  • Thorsten Mascher (Ludwig-Maximilians-University Munich)

• 12:00-12:20 – SepF is a New Membrane Anchor for the Key Cell Division Protein FtsZ
  • Ilkay Celik (Newcastle University)

• 12:20-12:40 – The Bacterial MreB Cytoskeleton Organizes the Cell Membrane
  • Henrik Strahl (Newcastle University)

• 12:40 – 14:20 LUNCH (+ BACIP MEETING)

Tuesday April 24th 2012 – Afternoon

Session III: Adaptation, Sporulation and DNA Replication

CHAIR: Ulrike Mäder

• 14:20-14:40 – Selecting Better Bet Hedging Bacillus Strains
  • Jeroen Siebring (University of Groningen)

• 14:40-15:00 – Spore Coat Proteins: Self-Assembly Properties and Protein-Protein Interactions
  • Daniela Krajčíková (Slovak Academy of Sciences)

• 15:00-15:20 – Spo0A links DNA Replication with Sporulation by Direct Binding to the Origin of Replication in Bacillus subtilis
  • Mirjam Boonstra (University of Groningen)

• • 15:20-15:40 – DSB Repair Pathway is Required for Viability of the SMC-ScpA-ScpB Depleted Strain
  • Etienne Dervyn (Institut Micalis, INRA)

• 15:40-16:10 - COFFEE BREAK

Session IV: Antibiotics

CHAIR: Eugenio Ferrari

• 16:10-16:30 – Regulated Expression of a Multidrug-ABC Transporter – The yheH-yheI-yheJ Operon
  • Ewoud Reilman (University of Groningen)

• 16:30-16:50 – Signal Transduction in Antimicrobial Peptide Detoxification Modules of Bacillus subtilis: Interaction between ABC Transport and Sensor Kinase
  • Susanne Gebhard (Ludwig-Maximilians-University Munich)

• 16:50-17:10 – Structure-Function Relationship of the Lantibiotic Immunity-Protein SpaI
  • Sophie Bochmann (Goethe University Frankfurt)

• 17:10-17:30 – Structure, Biosynthesis and Properties of Kurstakins, Nonribosomal Lipopeptides from Bacillus spp.
  • Philippe Jacques (Universite de Lille)

Wednesday April 25th 2012 - Morning

Session V: Application

CHAIR: Jörg Stülke

• 9:00-9:20 – Bacillus subtilis Cell Factory Producing Scyllo-Inositol, A Potential Therapeutic Agent for Alzheimer’s Disease
  • Ken-ichi Yoshida (Kobe University)

• 9:20-9:40 – Developing an Enzyme Discovery Tool for Bacillus subtilis
  • Tjeerd van Rij (DSM)

• 9:40 – 10:00 - Use of Bacillus amyloliquefaciens for Control of Plant Stress
  • Johan Meijer (University of Uppsala)

• 10:00 – 10:20 – Phenotypic Heterogeneity during Heterologous Protein Production in Bacillus megaterium
  • Rebekka Biedendieck (Technische Universität Braunschweig)

• 10:20-10:40 – Absolute Protein Quantification - Application in Bacillus subtilis
  • Dörte Becher (Ernst-Moritz-Arndt Universität Greifswald)

10:40-11:00 - COFFEE BREAK

Session VI: RNA Metabolism and Regulation

CHAIR: Uwe Völker

• 11:00-11:20 – Three Essential Ribonucleases RNase Y, J1 and III Control the Abundance of a Majority of B. subtilis mRNAs
  • Ciarán Condon (Institut de Biologie Physico-Chimique CNRS)

• 11:20-11:40 – The Effects of RpoE and HelD on RNA Polymerase from Bacillus subtilis
  • Libor Krásný (Academy of Sciences of the Czech Republic)

• 11:40 – 12:00 – Dynamic Localization of an RNA-Binding Transcription Factor in Bacillus subtilis
  • Fabian Rothe (University of Göttingen)

• 12:00 – 12:20 – Requirements for the High-Frequency Activation of the Cryptic gudB Glutamate Dehydrogenase Gene in Bacillus subtilis
  • Fabian Commichau (Georg-August-Universität Göttingen)

• 12:20-12:40 – The Anaerobic Formation of Acetoin Regulated by the Transcriptional Regulator AlsR – Part of pH Homeostasis in Bacillus subtilis
  • Elisabeth Härtig (TU Braunschweig)

12:40-14:00 – LUNCH

Wednesday April 25th 2012 - Afternoon

EU BaSynthec Synthetic Biology Symposium

CHAIR: Kevin Devine

• 14:00-14:10 – Introduction – Philippe Noirot (INRA)
• 14:10-14:40 – Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis - Naotake Ogasawara (Nara Institute)
• 14:40-15:10 – Computational and Bioinformatics Approaches to Enabling Synthetic Biology - Anil Wipat (Newcastle University)
• 15:10-15:40 – Highly Modified Peptides by Synthetic Biology Approaches - Oscar Kuipers (Groningen University)

• 15:40-16:10 – COFFEE BREAK

• 16:10-16:40 – Bacillus subtilis Minimal Genome Work in Europe - Philippe Noirot (INRA)
• 16:40-17:10 – View of Novozymes on Synthetic Biology - Allen Kent Nielsen (Novozymes)
• 17:10-17:40 – View of DSM on Synthetic Biology - Zoltán Prágai (DSM)
• 17:40-17:50 – Wrap up / end of workshop – Philippe Noirot (INRA)