Difference between revisions of "SucC"

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(Biological materials)
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A1006 ( ''sucC''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1006&Search=1A1006 BGSC]
 
** 1A1006 ( ''sucC''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1006&Search=1A1006 BGSC]
** GP1134 (cat), available in [[Stülke]] lab
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** GP1134 (cat), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 16:11, 30 October 2012

  • Description: succinyl-CoA synthetase (beta subunit)

Gene name sucC
Synonyms
Essential no
Product succinyl-CoA synthetase (beta subunit)
Function TCA cycle
Gene expression levels in SubtiExpress: sucC
Interactions involving this protein in SubtInteract: SucC
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 41 kDa, 4.846
Gene length, protein length 1155 bp, 385 aa
Immediate neighbours ylqH, sucD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SucC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SucC expression.png















Categories containing this gene/protein

ATP synthesis, carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU16090

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
  • Protein family: ATP-grasp domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Michaelis-Menten FEBS Letters
  • Domains:
  • Modification: phosphorylation on Ser-220 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1JKJ (E. coli)
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia

Expression and regulation

  • Sigma factor:
  • Regulation: repressed by glucose (2.7-fold) (CcpA) PubMed
  • Regulatory mechanism: CcpA: transcription repression PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References