YkuI

• Description: potential c-di-GMP degrading phosphodiesterase
  
  

Gene name	ykuI
Synonyms
Essential	no
Product	potential c-di-GMP degrading phosphodiesterase
Function	unknown
Gene expression levels in SubtiExpress: ykuI
MW, pI	47 kDa, 4.462
Gene length, protein length	1221 bp, 407 aa
Immediate neighbours	ykuH, fsrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU14090

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: degradation of cyclic di-GMP PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: EAL domain (aa 1 ... 245, for c-di-GMP hydrolysis), PAS-like domain (aa 300 ... 400) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BAS, in complex with c-di-GMP 2W27 PubMed

• UniProt: O35014

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykuI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1324 (tet), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

George Minasov, Sivaraman Padavattan, Ludmilla Shuvalova, Joseph S Brunzelle, Darcie J Miller, Arnaud Baslé, Claudia Massa, Frank R Collart, Tilman Schirmer, Wayne F Anderson
Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains.
J Biol Chem: 2009, 284(19);13174-84
[PubMed:19244251] [WorldCat.org] [DOI] (P p)