Difference between revisions of "TsaD"

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m (moved Gcp to TsaD: 23072323)
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* '''Description:''' there are two annotations for the corresponding ''E. coli'' protein: O-sialoglycoprotein endopeptidase, and required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with [[YwlC]]), [[universally conserved protein]] <br/><br/>
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* '''Description:''' required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with [[TsaC]]), [[universally conserved protein]] <br/><br/>
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''gcp''
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|''tsaD''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydiE ''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydiE, gcp ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification  
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05940 gcp]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05940 tsaD]
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Gcp Gcp]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/TsaD TsaD]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.016   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.016   
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=== Additional information===
 
=== Additional information===
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* '''Catalyzed reaction/ biological activity:'''
 
* '''Catalyzed reaction/ biological activity:'''
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** biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs {{PubMed|21183954}}
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** biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([[TsaB]]-[[TsaD]]-[[TsaE]]) {{PubMed|23072323,21183954}}
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** conserved glycopeptidase that may be directly involved in the metabolism of glycated proteins {{PubMed|20824107}}
 
  
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* '''Protein family:''' peptidase M22 family (according to Swiss-Prot)
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* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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** the corresponding ''E. coli'' protein interacts with the proteins corresponding to [[YdiB]] and [[YdiC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19376873 PubMed]
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** [[TsaB]]-[[TsaD]] {{PubMed|23072323,21285948}}
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** [[Gcp]]-[[YdiC]] {{PubMed|21285948}}
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** [[TsaD]]-[[TsaE]] {{PubMed|23072323}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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=References=
 
=References=
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<pubmed> 23072323 </pubmed>
 
==Publications on the corresponding'' E. coli'' protein, YgjD==
 
==Publications on the corresponding'' E. coli'' protein, YgjD==
 
<pubmed> 21183954 19376873 20824107 21619589 21285948 </pubmed>
 
<pubmed> 21183954 19376873 20824107 21619589 21285948 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:13, 18 October 2012

  • Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein

Gene name tsaD
Synonyms ydiE, gcp
Essential yes PubMed
Product tRNA modification enzyme
Function tRNA modification
Gene expression levels in SubtiExpress: tsaD
Interactions involving this protein in SubtInteract: TsaD
MW, pI 36 kDa, 5.016
Gene length, protein length 1038 bp, 346 aa
Immediate neighbours ydiD, ydiF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Gcp context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Gcp expression.png















Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU05940

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

universally conserved protein

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Publications on the corresponding E. coli protein, YgjD

Tobias Bergmiller, Rafael Peña-Miller, Alexander Boehm, Martin Ackermann
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
BMC Microbiol: 2011, 11;118
[PubMed:21619589] [WorldCat.org] [DOI] (I e)

Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948] [WorldCat.org] [DOI] (I p)

Madhusudhan Srinivasan, Preeti Mehta, Yao Yu, Evelyn Prugar, Eugene V Koonin, A Wali Karzai, Rolf Sternglanz
The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A.
EMBO J: 2011, 30(5);873-81
[PubMed:21183954] [WorldCat.org] [DOI] (I p)

Chen Katz, Ifat Cohen-Or, Uri Gophna, Eliora Z Ron
The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
mBio: 2010, 1(3);
[PubMed:20824107] [WorldCat.org] [DOI] (I e)

Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873] [WorldCat.org] [DOI] (I p)

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