XylB

• Description: xylulokinase
  
  

Gene name	xylB
Synonyms	yncA
Essential	no
Product	xylulokinase
Function	utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	55 kDa, 6.459
Gene length, protein length	1497 bp, 499 aa
Immediate neighbours	xylA, yncB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

• Locus tag: BSU17610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate (according to Swiss-Prot)

• Protein family: FGGY kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2NLX (from E. coli, 35% identity, 52% similarity) PubMed

• UniProt: P39211

• KEGG entry: [3]

• E.C. number: 2.7.1.17

Expression and regulation

• Operon: xylA-xylB PubMed

• Expression browser: xylB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by xylose (XylR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • XylR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1151 (del aphA3) available in Stülke lab
  • 1A719 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage

References

Eric Di Luccio, Barbara Petschacher, Jennifer Voegtli, Hui-Ting Chou, Henning Stahlberg, Bernd Nidetzky, David K Wilson
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli.
J Mol Biol: 2007, 365(3);783-98
[PubMed:17123542] [WorldCat.org] [DOI] (P p)

A Kraus, C Hueck, D Gärtner, W Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
J Bacteriol: 1994, 176(6);1738-45
[PubMed:8132469] [WorldCat.org] [DOI] (P p)

S Jacob, R Allmansberger, D Gärtner, W Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Mol Gen Genet: 1991, 229(2);189-96
[PubMed:1921970] [WorldCat.org] [DOI] (P p)

D Gärtner, M Geissendörfer, W Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
J Bacteriol: 1988, 170(7);3102-9
[PubMed:2454911] [WorldCat.org] [DOI] (P p)