CysJ

• Description: sulfite reductase (NADPH2)
  
  

Gene name	cysJ
Synonyms	yvgQ
Essential	no
Product	sulfite reductase (NADPH2)
Function	sulfite reduction
Gene expression levels in SubtiExpress: cysJ
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	64 kDa, 5.49
Gene length, protein length	1713 bp, 571 aa
Immediate neighbours	nhaK, cysI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CysL regulon

The gene

Basic information

• Locus tag: BSU33430

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32214

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cysI-cysJ PubMed

• Expression browser: cysJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • CysL: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A802 ( cysJ::kan), PubMed, available at BGSC
  • 1A934 ( cysJ::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: