Difference between revisions of "Hag"
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* '''Mutant:''' GP901 (aphA3), GP902 (tet), both available in [[Stülke]] lab | * '''Mutant:''' GP901 (aphA3), GP902 (tet), both available in [[Stülke]] lab | ||
+ | ** 1A915 ( ''hag''::''cat''), {{PubMed|19202088}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A915&Search=1A915 BGSC] | ||
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 09:07, 19 September 2012
- Description: flagellin protein
Gene name | hag |
Synonyms | |
Essential | no |
Product | flagellin protein |
Function | motility and chemotaxis |
Gene expression levels in SubtiExpress: hag | |
Interactions involving this protein in SubtInteract: Hag | |
MW, pI | 32 kDa, 4.782 |
Gene length, protein length | 912 bp, 304 aa |
Immediate neighbours | yvyC, csrA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
CodY regulon, CsrA regulon, ScoC regulon, SigD regulon
The gene
Basic information
- Locus tag: BSU35360
Phenotypes of a mutant
No swarming motility on B medium. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: bacterial flagellin family (according to Swiss-Prot)
- Paralogous protein(s): YvzB (C-terminal domain of Hag)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P02968
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
- lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab
- GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Stülke lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Additional Reviews: PubMed
Original Publications
Additional publications: PubMed
Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects Flagellin Expression and Biofilm Formation. J Bacteriol.: 2011, 193(21):5997-6007. PubMed:21856853