Difference between revisions of "GltB"
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[[Stülke|Jörg Stülke]], University of Göttingen, Germany | [[Stülke|Jörg Stülke]], University of Göttingen, Germany | ||
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage] | [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage] | ||
+ | |||
+ | [[Fabian_Commichau|Fabian Commichau]] University of Göttingen, Germany | ||
+ | [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage] | ||
=Your additional remarks= | =Your additional remarks= |
Revision as of 19:07, 18 September 2012
- Description: small subunit of glutamate synthase
Gene name | gltB |
Synonyms | |
Essential | no |
Product | glutamate synthase (small subunit) |
Function | glutamate biosynthesis |
Gene expression levels in SubtiExpress: gltB | |
Interactions involving this protein in SubtInteract: GltB | |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
MW, pI | 54.6 kDa, 7.69 |
Gene length, protein length | 1479 bp, 493 amino acids |
Immediate neighbours | yogA, gltA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, glutamate metabolism
This gene is a member of the following regulons
GltC regulon, FsrA regulon, TnrA regulon
The gene
Basic information
- Locus tag: BSU18440
Phenotypes of a mutant
auxotrophic for glutamate
Database entries
- DBTBS entry: [1]
- SubtiList entry:[2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
- Protein family: glutamate synthase family.
- Paralogous protein(s): none
Extended information on the protein
- Kinetic information:
- Domains:
- nucleotide binding domain (NADP) (299–313)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: O34399
- KEGG entry: [3]
- E.C. number: 1.4.1.13
Additional information
Expression and regulation
- Regulation: see gltA
- Additional information:
Biological materials
- Mutant: GP807 (del gltAB::tet), GP517 (ermC), both available in Stülke lab
- Expression vector:
- lacZ fusion: see gltA
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Fabian Commichau University of Göttingen, Germany Homepage
Your additional remarks
References
Reviews
Gunka K, Commichau FM Control of glutamate homeostasis in Bacillus subtilis: a complex interplay between ammonium assimilation, glutamate biosynthesis and degradation. Mol Microbiol.: 2012, 85(2) 213-224. PubMed:22625175
Original publications