Difference between revisions of "CheD"
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| − | * '''Description:''' required for methylation of methyl-accepting chemotaxis proteins by [[CheR]], enhances phosphatase activity of [[CheC]] <br/><br/> | + | * '''Description:''' protein deaminase, required for methylation of methyl-accepting chemotaxis proteins by [[CheR]], enhances phosphatase activity of [[CheC]], required for full [[McpC]] activity <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || [[CheC]] activity modulator | + | |style="background:#ABCDEF;" align="center"| '''Product''' || protein deaminase, [[CheC]] activity modulator |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]] | |style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]] | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Protein L-glutamine + H<sub>2</sub>O = protein L-glutamate + NH<sub>3</sub> (according to Swiss-Prot) | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** Protein L-glutamine + H<sub>2</sub>O = protein L-glutamate + NH<sub>3</sub> (according to Swiss-Prot) | ||
| + | ** deaminates Gln-586, Gln-593, and Gln594 in [[McpA]] {{PubMed|22931217}} | ||
| + | ** deaminates Gln-609 in [[McpC]] {{PubMed|22931217}} | ||
* '''Protein family:''' cheD family (according to Swiss-Prot) | * '''Protein family:''' cheD family (according to Swiss-Prot) | ||
Revision as of 12:54, 31 August 2012
- Description: protein deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity
| Gene name | cheD |
| Synonyms | ylxK |
| Essential | no |
| Product | protein deaminase, CheC activity modulator |
| Function | motility and chemotaxis |
| Gene expression levels in SubtiExpress: cheD | |
| Interactions involving this protein in SubtInteract: CheD | |
| MW, pI | 17 kDa, 9.347 |
| Gene length, protein length | 498 bp, 166 aa |
| Immediate neighbours | cheC, sigD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
CodY regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cheD family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P40404
- KEGG entry: [3]
- E.C. number: 3.5.1.44
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
- in minimal medium, CheD is present with 1,200 +/- 250 molecules per cell PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
