Difference between revisions of "ComX"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || competence pheromone precursor | |style="background:#ABCDEF;" align="center"| '''Product''' || competence pheromone precursor | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || quorum sensing | + | |style="background:#ABCDEF;" align="center"|'''Function''' || [[quorum sensing]] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31700 comX] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31700 comX] | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/ComX ComX] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 4.358 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 4.358 | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
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=== Additional information=== | === Additional information=== | ||
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* '''Domains:''' | * '''Domains:''' | ||
| − | * '''Modification:''' is synthesized as an inactive precursor, cleaved, and modified ( | + | * '''Modification:''' is synthesized as an inactive precursor, cleaved, and modified (prenylated) by [[ComQ]] prior to excretion {{PubMed|22878193}} |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[ComQ]]-[[ComX]] {{PubMed|22878193}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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==Original publications== | ==Original publications== | ||
'''Additional publications:''' {{PubMed|22511326,21636272}} | '''Additional publications:''' {{PubMed|22511326,21636272}} | ||
| − | <pubmed>16407988,11133937,12067344,19202088,14679219,15564679,17240141,19605685, 1715859 </pubmed> | + | <pubmed>16407988,11133937,12067344,19202088,14679219,15564679,17240141,19605685, 1715859 22878193</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:25, 12 August 2012
- Description: competence pheromone precursor, transfers cell density signal to ComP; triggers the production of surfactin
| Gene name | comX |
| Synonyms | |
| Essential | no |
| Product | competence pheromone precursor |
| Function | quorum sensing |
| Gene expression levels in SubtiExpress: comX | |
| Interactions involving this protein in SubtInteract: ComX | |
| MW, pI | 6 kDa, 4.358 |
| Gene length, protein length | 165 bp, 55 aa |
| Immediate neighbours | comP, comQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
genetic competence, quorum sensing
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31700
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: is synthesized as an inactive precursor, cleaved, and modified (prenylated) by ComQ prior to excretion PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P45453
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
The ComX --> surfactin signaling pathway is an example for paracrine signaling in bacteria
References
Reviews
Elizabeth Anne Shank, Roberto Kolter
Extracellular signaling and multicellularity in Bacillus subtilis.
Curr Opin Microbiol: 2011, 14(6);741-7
[PubMed:22024380]
[WorldCat.org]
[DOI]
(I p)
Original publications
Additional publications: PubMed
Fumitada Tsuji, Ayako Ishihara, Aya Nakagawa, Masahiro Okada, Shigeyuki Kitamura, Kyoko Kanamaru, Yuichi Masuda, Kazuma Murakami, Kazuhiro Irie, Youji Sakagami
Lack of the consensus sequence necessary for tryptophan prenylation in the ComX pheromone precursor.
Biosci Biotechnol Biochem: 2012, 76(8);1492-6
[PubMed:22878193]
[WorldCat.org]
[DOI]
(I p)
Daniel López, Hera Vlamakis, Richard Losick, Roberto Kolter
Paracrine signaling in a bacterium.
Genes Dev: 2009, 23(14);1631-8
[PubMed:19605685]
[WorldCat.org]
[DOI]
(I p)
Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088]
[WorldCat.org]
[DOI]
(P p)
Masahiro Okada, Hisao Yamaguchi, Isao Sato, Soo Jeong Cho, David Dubnau, Youji Sakagami
Structure-activity relationship studies on quorum sensing ComX(RO-E-2) pheromone.
Bioorg Med Chem Lett: 2007, 17(6);1705-7
[PubMed:17240141]
[WorldCat.org]
[DOI]
(P p)
Masahiro Okada, Isao Sato, Soo Jeong Cho, Hidehisa Iwata, Toshihiko Nishio, David Dubnau, Youji Sakagami
Structure of the Bacillus subtilis quorum-sensing peptide pheromone ComX.
Nat Chem Biol: 2005, 1(1);23-4
[PubMed:16407988]
[WorldCat.org]
[DOI]
(P p)
Masahiro Okada, Isao Sato, Soo Jeong Cho, Yoshihiro Suzuki, Makoto Ojika, David Dubnau, Youji Sakagami
Towards structural determination of the ComX pheromone: synthetic studies on peptides containing geranyltryptophan.
Biosci Biotechnol Biochem: 2004, 68(11);2374-87
[PubMed:15564679]
[WorldCat.org]
[DOI]
(P p)
M Ansaldi, D Dubnau
Diversifying selection at the Bacillus quorum-sensing locus and determinants of modification specificity during synthesis of the ComX pheromone.
J Bacteriol: 2004, 186(1);15-21
[PubMed:14679219]
[WorldCat.org]
[DOI]
(P p)
Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344]
[WorldCat.org]
[DOI]
(P p)
P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937]
[WorldCat.org]
[DOI]
(P p)
Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859]
[WorldCat.org]
[DOI]
(P p)
