PhrG

• Description: response regulator aspartate phosphatase (RapG) regulator
  
  

Gene name	phrG
Synonyms	yycL
Essential	no
Product	phosphatase (RapG) regulator
Function	control of DegU activity
Gene expression levels in SubtiExpress: phrG
Interactions involving this protein in SubtInteract: PhrG
MW, pI	4 kDa, 9.017
Gene length, protein length	114 bp, 38 aa
Immediate neighbours	rapG, rocF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

CcpA regulon, RghR regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU40310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to RapG, this results in the inability of RapG to interact with DegU PubMed

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PhrG-RapG PubMed

• Localization:

Database entries

• Structure:

• UniProt: O32295

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapG-phrG PubMed

• Expression browser: phrG PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • repressed by SinR PubMed
  • repressed by RghR PubMed
  • repressed by glucose (4.8-fold) (CcpA) PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed
  • RghR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kentaro Hayashi, Tsukahara Kensuke, Kazuo Kobayashi, Naotake Ogasawara, Mitsuo Ogura
Bacillus subtilis RghR (YvaN) represses rapG and rapH, which encode inhibitors of expression of the srfA operon.
Mol Microbiol: 2006, 59(6);1714-29
[PubMed:16553878] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Kana Shimane, Kei Asai, Naotake Ogasawara, Teruo Tanaka
Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1685-97
[PubMed:12950930] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)