DnaB

• Description: initiation of chromosome replication/ membrane attachment protein, part of the replisome
  
  

Gene name	dnaB
Synonyms
Essential	yes PubMed
Product	initiation of chromosome replication/ membrane attachment protein
Function	DNA replication
Gene expression levels in SubtiExpress: dnaB
Interactions involving this protein in SubtInteract: DnaB
MW, pI	54 kDa, 5.278
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	dnaI, ytcG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU28990

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-PriA PubMed
  • DnaB-DnaI PubMed
  • DnaB-DnaC PubMed
  • DnaD-DnaB PubMed
  • DnaB-DnaX PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization:
  • Membrane-proximal (Spotty) PubMed
  • forms foci, close to oriC PubMed

Database entries

• Structure:

• UniProt: P07908

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dnaB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)