DnaD

• Description: initiation of chromosome replication, part of the replisome
  
  

Gene name	dnaD
Synonyms
Essential	yes PubMed
Product	initiation of chromosome replication
Function	DNA replication
Gene expression levels in SubtiExpress: dnaD
Interactions involving this protein in SubtInteract: DnaD
MW, pI	27 kDa, 7.174
Gene length, protein length	696 bp, 232 aa
Immediate neighbours	nth, asnS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU22350

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • DnaD-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
  • required for the assembly of the replicative helicase DnaC at the origin of replication PubMed
  • interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA PubMed

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaD-PriA PubMed
  • DnaD-DnaA PubMed
  • DnaD-DnaB PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• Structure: 2C79 (N-terminal domain)

• UniProt: P39787

• KEGG entry: [2]

• E.C. number:

Expression and regulation

Operon: dnaD-nth PubMed 

• Expression browser: dnaD PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed
  • there are about 3,000 to 5,000 molecules of DnaD per cell PubMed
  • expression is transiently increased after treatment with H2O2 PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Alan Grossman, MIT, Cambridge, MA, USA

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)