BlyA

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	blyA
Synonyms	yomC
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	SPß-mediated lysis
Gene expression levels in SubtiExpress: blyA
MW, pI	39 kDa, 9.935
Gene length, protein length	1101 bp, 367 aa
Immediate neighbours	yomD, bhlA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, SP-beta prophage

The gene

Basic information

• Locus tag: BSU21410

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_2 domain (like CwlA, CwlH, XlyA, XlyB)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31982

• KEGG entry: [2]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:

• Expression browser: blyA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexandre Regamey, Dimitri Karamata
The N-acetylmuramoyl-L-alanine amidase encoded by the Bacillus subtilis 168 prophage SP beta.
Microbiology (Reading): 1998, 144 ( Pt 4);885-893
[PubMed:9579063] [WorldCat.org] [DOI] (P p)