UxaB

Gene name	uxaB
Synonyms	yjmI
Essential	no
Product	tagaturonate reductase
Function	hexuronate utilization
Gene expression levels in SubtiExpress: uxaB
MW, pI	55 kDa, 5.056
Gene length, protein length	1440 bp, 480 aa
Immediate neighbours	exuR, uxaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, sporulation proteins

This gene is a member of the following regulons

CcpA regulon, ExuR regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU12380

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-altronate + NAD⁺ = D-tagaturonate + NADH (according to Swiss-Prot)

• Protein family: UxaB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34354

• KEGG entry: [3]

• E.C. number: 1.1.1.58

Expression and regulation

• Operon:
  • uxaC-yjmB-yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA PubMed
  • yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA PubMed

• Expression browser: uxaB PubMed

• Sigma factor:
  • uxaC: SigA PubMed
  • yjmC: SigE PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed1 PubMed2
  • induced by galacturonate (ExuR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed1 PubMed2
  • ExuR: reanscription repression in the absence of inducer PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)

K R Mekjian, E M Bryan, B W Beall, C P Moran
Regulation of hexuronate utilization in Bacillus subtilis.
J Bacteriol: 1999, 181(2);426-33
[PubMed:9882655] [WorldCat.org] [DOI] (P p)

Carlo Rivolta, Blazenka Soldo, Vladimir Lazarevic, Bernard Joris, Catherine Mauël, Dimitri Karamat
A 35.7 kb DNA fragment from the Bacillus subtilis chromosome containing a putative 12.3 kb operon involved in hexuronate catabolism and a perfectly symmetrical hypothetical catabolite-responsive element.
Microbiology (Reading): 1998, 144 ( Pt 4);877-884
[PubMed:9579062] [WorldCat.org] [DOI] (P p)