YesX

Gene name	yesX
Synonyms
Essential	no
Product	rhamnogalacturonan lyase, degrades oligo- to disaccharides
Function	utilization of rhamnogalacturonan
Gene expression levels in SubtiExpress: yesX
MW, pI	67 kDa, 5.223
Gene length, protein length	1836 bp, 612 aa
Immediate neighbours	yesW, yesY
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YesS regulon

The gene

Basic information

• Locus tag: BSU07060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: polysaccharide lyase 11 family (according to Swiss-Prot)

• Paralogous protein(s): YesW

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure: 2ZUY

• UniProt: O31527

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yesS-yesT-yesU-yesV-yesW-yesX-yesY-yesZ PubMed

• Expression browser: yesX PubMed

• Sigma factor:

• Regulation: induced by pectin (YesS) PubMed

• Regulatory mechanism: YesS: transcriptional activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sandrine Poncet, Maryline Soret, Peggy Mervelet, Josef Deutscher, Philippe Noirot
Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system.
J Biol Chem: 2009, 284(41);28188-28197
[PubMed:19651770] [WorldCat.org] [DOI] (I p)

Akihito Ochiai, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J Biol Chem: 2009, 284(15);10181-9
[PubMed:19193638] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)