Difference between revisions of "PGP1871"

Revision as of 12:26, 1 August 2012

pGP1871: click to enlarge

General comments

Integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor

Hints for the usage

amplify the last 600bp of your gene of interest,
skip the Stopp-Codon of the gene in the reverse primer
take care that the integration is in-frame
do not use the HindIII site for cloning, as GFP is inserted via this restriction site

Revision as of 12:33, 3 November 2011 (view source) MLehnik (talk \| contribs) (Created page with "250px\|thumb\|right\|'''pGP1871: click to enlarge''' '''General comments''' Integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, ...")	Revision as of 12:26, 1 August 2012 (view source) Jstuelk (talk \| contribs) m (moved Pgp1871 to PGP1871) Newer edit →
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