Difference between revisions of "CotE"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat morphogenetic protein | |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat morphogenetic protein | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || assembly of | + | |style="background:#ABCDEF;" align="center"|'''Function''' || assembly of the outer spore coat |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CotE CotE] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CotE CotE] | ||
| Line 35: | Line 35: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
| Line 46: | Line 42: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[SigE regulon]]}}, | {{SubtiWiki regulon|[[SigE regulon]]}}, | ||
| − | {{SubtiWiki regulon|[[SigK regulon]]}} | + | {{SubtiWiki regulon|[[SigK regulon]]}}, |
| + | {{SubtiWiki regulon|[[GerE regulon]]}} | ||
=The gene= | =The gene= | ||
| Line 55: | Line 52: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * mis-assembly of the outer spore coat {{PubMed|22171814}} | ||
=== Database entries === | === Database entries === | ||
| Line 69: | Line 67: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' assembly of the [[CotC]]-[[CotU]] complex {{PubMed|20023017,18065538}} | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** assembly of the [[CotC]]-[[CotU]] complex {{PubMed|20023017,18065538}} | ||
* '''Protein family:''' cotE family (according to Swiss-Prot) | * '''Protein family:''' cotE family (according to Swiss-Prot) | ||
| Line 95: | Line 94: | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
** encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore {{PubMed|22463703}} | ** encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore {{PubMed|22463703}} | ||
| − | ** spore coat {{PubMed|22773792}} | + | ** spore coat {{PubMed|22773792,22171814}} |
=== Database entries === | === Database entries === | ||
| Line 119: | Line 118: | ||
* '''Regulation:''' | * '''Regulation:''' | ||
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
| − | ** expressed during sporulation in the mother cell ([[SigE]], [[SigK]]) {{PubMed|1691789}} | + | ** expressed during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]]) {{PubMed|1691789,15383836}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[GerE]]: transcription repression {{PubMed|15383836}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 149: | Line 149: | ||
=References= | =References= | ||
'''Additional publications:''' {{PubMed|22463703,21821751}} | '''Additional publications:''' {{PubMed|22463703,21821751}} | ||
| − | <pubmed>8748030,17720779,10559171 8760914 15699190 7592342,3139490, 8299942,1691789, 12644503, 17172339,19304857,11737650 ,12107147, 20023017 18065538 22773792</pubmed> | + | <pubmed>8748030,17720779,10559171 8760914 15699190 7592342,3139490, 8299942,1691789, 12644503, 17172339,19304857,11737650 ,12107147, 20023017 18065538 22773792 15383836 22171814</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:38, 29 July 2012
- Description: outer spore coat morphogenetic protein
| Gene name | cotE |
| Synonyms | |
| Essential | no |
| Product | spore coat morphogenetic protein |
| Function | assembly of the outer spore coat |
| Interactions involving this protein in SubtInteract: CotE | |
| MW, pI | 20 kDa, 4.139 |
| Gene length, protein length | 543 bp, 181 aa |
| Immediate neighbours | ymcA, mutS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
SigE regulon, SigK regulon, GerE regulon
The gene
Basic information
- Locus tag: BSU17030
Phenotypes of a mutant
- mis-assembly of the outer spore coat PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Protein family: cotE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P14016
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
Adriano Henriques, Lisbon, Portugal homepage
Your additional remarks
References
Additional publications: PubMed
Melissa de Francesco, Jake Z Jacobs, Filipa Nunes, Mónica Serrano, Peter T McKenney, Ming-Hsiu Chua, Adriano O Henriques, Patrick Eichenberger
Physical interaction between coat morphogenetic proteins SpoVID and CotE is necessary for spore encasement in Bacillus subtilis.
J Bacteriol: 2012, 194(18);4941-50
[PubMed:22773792]
[WorldCat.org]
[DOI]
(I p)
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Rachele Isticato, Assunta Pelosi, Maurilio De Felice, Ezio Ricca
CotE binds to CotC and CotU and mediates their interaction during spore coat formation in Bacillus subtilis.
J Bacteriol: 2010, 192(4);949-54
[PubMed:20023017]
[WorldCat.org]
[DOI]
(I p)
Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857]
[WorldCat.org]
[DOI]
(I p)
Rachele Isticato, Assunta Pelosi, Rita Zilhão, Loredana Baccigalupi, Adriano O Henriques, Maurilio De Felice, Ezio Ricca
CotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2008, 190(4);1267-75
[PubMed:18065538]
[WorldCat.org]
[DOI]
(I p)
Caitlin C Ferguson, Amy H Camp, Richard Losick
gerT, a newly discovered germination gene under the control of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(21);7681-9
[PubMed:17720779]
[WorldCat.org]
[DOI]
(P p)
Teresa Costa, Mónica Serrano, Leif Steil, Uwe Völker, Charles P Moran, Adriano O Henriques
The timing of cotE expression affects Bacillus subtilis spore coat morphology but not lysozyme resistance.
J Bacteriol: 2007, 189(6);2401-10
[PubMed:17172339]
[WorldCat.org]
[DOI]
(P p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503]
[WorldCat.org]
[DOI]
(P p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
S Little, A Driks
Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.
Mol Microbiol: 2001, 42(4);1107-20
[PubMed:11737650]
[WorldCat.org]
[DOI]
(P p)
T Bauer, S Little, A G Stöver, A Driks
Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE.
J Bacteriol: 1999, 181(22);7043-51
[PubMed:10559171]
[WorldCat.org]
[DOI]
(P p)
F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914]
[WorldCat.org]
[DOI]
(P p)
K Pogliano, E Harry, R Losick
Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy.
Mol Microbiol: 1995, 18(3);459-70
[PubMed:8748030]
[WorldCat.org]
[DOI]
(P p)
C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342]
[WorldCat.org]
[DOI]
(P p)
A Driks, S Roels, B Beall, C P Moran, R Losick
Subcellular localization of proteins involved in the assembly of the spore coat of Bacillus subtilis.
Genes Dev: 1994, 8(2);234-44
[PubMed:8299942]
[WorldCat.org]
[DOI]
(P p)
L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789]
[WorldCat.org]
[DOI]
(P p)
L B Zheng, W P Donovan, P C Fitz-James, R Losick
Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore.
Genes Dev: 1988, 2(8);1047-54
[PubMed:3139490]
[WorldCat.org]
[DOI]
(P p)
