Difference between revisions of "CdaR"
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP999 (''[[ybbR]]''::''cat''), available in [[Stülke]] lab | + | ** GP999 (''[[ybbR]]''::''cat''), available in [[Jörg Stülke]]'s lab |
| − | ** GP985 (''[[ybbP]]''-''[[ybbR]]''::''cat''), available in [[Stülke]] lab | + | ** GP985 (''[[ybbP]]''-''[[ybbR]]''::''cat''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 130: | Line 130: | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab. Respective plasmids: pGP1989 and pGP1992. | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab. Respective plasmids: pGP1989 and pGP1992. |
| − | * '''Strep-tag construct:''' GP1330 (aphA3, based on [[pGP1460]]), available in | + | * '''Strep-tag construct:''' GP1330 (aphA3, based on [[pGP1460]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 05:50, 29 June 2012
- Description: unknown
| Gene name | ybbR |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | unknown |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 52 kDa, 4.719 |
| Gene length, protein length | 1449 bp, 483 aa |
| Immediate neighbours | ybbP, glmM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01760
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34659
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- GP999 (ybbR::cat), available in Jörg Stülke's lab
- GP985 (ybbP-ybbR::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.
- Strep-tag construct: GP1330 (aphA3, based on pGP1460), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Jun Yin, Paul D Straight, Shaun M McLoughlin, Zhe Zhou, Alison J Lin, David E Golan, Neil L Kelleher, Roberto Kolter, Christopher T Walsh
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
Proc Natl Acad Sci U S A: 2005, 102(44);15815-20
[PubMed:16236721]
[WorldCat.org]
[DOI]
(P p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
