Difference between revisions of "GanA"

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(Biological materials)
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* '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab
 
* '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 13:29, 30 April 2012

  • Description: beta-galactosidase

Gene name lacA
Synonyms yvfN
Essential no
Product beta-galactosidase
Function galactan utilization
MW, pI 79 kDa, 5.704
Gene length, protein length 2061 bp, 687 aa
Immediate neighbours yvfO, yvfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LacA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LacA expression.png
























Categories containing this gene/protein

utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

  • Locus tag: BSU34130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-387 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:GP1181 (aphA3) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References