Difference between revisions of "TrmFO"
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Revision as of 09:33, 19 April 2012
- Description: flavoprotein, tRNA:m(5)U-54 methyltransferase, glucose-inhibited division protein
| Gene name | trmFO |
| Synonyms | ylyC, gid |
| Essential | no |
| Product | tRNA:m(5)U-54 methyltransferase |
| Function | tRNA modification |
| MW, pI | 47 kDa, 5.767 |
| Gene length, protein length | 1305 bp, 435 aa |
| Immediate neighbours | topA, codV |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16130
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA
- Protein family: TrmFO subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): FAD
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P39815
- KEGG entry: [2]
- E.C. number: 2.1.1.74
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Djemel Hamdane, Manuela Argentini, David Cornu, Hannu Myllykallio, Stéphane Skouloubris, Gaston Hui-Bon-Hoa, Béatrice Golinelli-Pimpaneau
Insights into folate/FAD-dependent tRNA methyltransferase mechanism: role of two highly conserved cysteines in catalysis.
J Biol Chem: 2011, 286(42);36268-80
[PubMed:21846722]
[WorldCat.org]
[DOI]
(I p)
Djemel Hamdane, Stéphane Skouloubris, Hannu Myllykallio, Béatrice Golinelli-Pimpaneau
Expression and purification of untagged and histidine-tagged folate-dependent tRNA:m5U54 methyltransferase from Bacillus subtilis.
Protein Expr Purif: 2010, 73(1);83-9
[PubMed:20412857]
[WorldCat.org]
[DOI]
(I p)
Jaunius Urbonavicius, Stéphane Skouloubris, Hannu Myllykallio, Henri Grosjean
Identification of a novel gene encoding a flavin-dependent tRNA:m5U methyltransferase in bacteria--evolutionary implications.
Nucleic Acids Res: 2005, 33(13);3955-64
[PubMed:16027442]
[WorldCat.org]
[DOI]
(I e)
