Difference between revisions of "CsrA"

From SubtiWiki
Jump to: navigation, search
Line 104: Line 104:
 
* '''Operon:''' ''csrA'' {{PubMed|8969505}}
 
* '''Operon:''' ''csrA'' {{PubMed|8969505}}
  
* '''[[Sigma factor]]:'''  
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=csrA_3636046_3636270_-1 csrA] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 110: Line 112:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 07:34, 17 April 2012

  • Description: motility regulator, binds to the hag mRNA to inhibit its translation

Gene name csrA
Synonyms yviG
Essential no
Product motility regulator
Function control of hag translation
Interactions involving this protein in SubtInteract: CsrA
MW, pI 8 kDa, 6.09
Gene length, protein length 222 bp, 74 aa
Immediate neighbours hag, fliW
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CsrA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

RNA binding regulators, motility and chemotaxis

This gene is a member of the following regulons

The CsrA regulon: hag

The gene

Basic information

  • Locus tag: BSU35370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: csrA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • CsrA is sequestered by interaction with FliW (the two proteins form a conserved module in many bacteria) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP469 (spc), available in Stülke lab
  • Expression vector:
    • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab
  • lacZ fusion: pGP461 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

Sampriti Mukherjee, Helen Yakhnin, Dave Kysela, Josh Sokoloski, Paul Babitzke, Daniel B Kearns
CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.
Mol Microbiol: 2011, 82(2);447-61
[PubMed:21895793] [WorldCat.org] [DOI] (I p)

Helen Yakhnin, Pallavi Pandit, Tom J Petty, Carol S Baker, Tony Romeo, Paul Babitzke
CsrA of Bacillus subtilis regulates translation initiation of the gene encoding the flagellin protein (hag) by blocking ribosome binding.
Mol Microbiol: 2007, 64(6);1605-20
[PubMed:17555441] [WorldCat.org] [DOI] (P p)

Prajna R Kulkarni, Xiaohui Cui, Joshua W Williams, Ann M Stevens, Rahul V Kulkarni
Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri.
Nucleic Acids Res: 2006, 34(11);3361-9
[PubMed:16822857] [WorldCat.org] [DOI] (I e)

B Soldo, V Lazarevic, C Mauël, D Karamata
Sequence of the 305 degrees-307 degrees region of the Bacillus subtilis chromosome.
Microbiology (Reading): 1996, 142 ( Pt 11);3079-88
[PubMed:8969505] [WorldCat.org] [DOI] (P p)