Difference between revisions of "Hag"
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* '''Operon:''' ''[[hag]]'' {{PubMed|2498284}} | * '''Operon:''' ''[[hag]]'' {{PubMed|2498284}} | ||
− | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hag_3634987_3635901_-1 hag] {{PubMed|22383849}} |
+ | |||
+ | * '''Sigma factor:''' [[SigD]] {{PubMed|2498284}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
− | ** the ''[[hag]]'' gene is strongly overexpressed in a ''[[ymdB]]'' mutant (loss of bistable gene expression) {{PubMed|21856853}} | + | ** the ''[[hag]]'' gene is strongly overexpressed in a ''[[ymdB]]'' mutant (loss of bistable gene expression) {{PubMed|21856853}} |
=Biological materials = | =Biological materials = |
Revision as of 07:34, 17 April 2012
- Description: flagellin protein
Gene name | hag |
Synonyms | |
Essential | no |
Product | flagellin protein |
Function | motility and chemotaxis |
Interactions involving this protein in SubtInteract: Hag | |
MW, pI | 32 kDa, 4.782 |
Gene length, protein length | 912 bp, 304 aa |
Immediate neighbours | yvyC, csrA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
CodY regulon, CsrA regulon, ScoC regulon, SigD regulon
The gene
Basic information
- Locus tag: BSU35360
Phenotypes of a mutant
No swarming motility on B medium. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: bacterial flagellin family (according to Swiss-Prot)
- Paralogous protein(s): YvzB (C-terminal domain of Hag)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P02968
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
- lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab
- GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Stülke lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects Flagellin Expression and Biofilm Formation. J Bacteriol.: 2011, 193(21):5997-6007. PubMed:21856853