Difference between revisions of "CysS"

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m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk)
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* '''Operon:''' ''[[gltX]]-[[cysE]]-[[cysS]]-[[mrnC]]-[[yacO]]-[[yacP]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
 
* '''Operon:''' ''[[gltX]]-[[cysE]]-[[cysS]]-[[mrnC]]-[[yacO]]-[[yacP]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysS_113450_114850_1 cysS] {{PubMed|22383849}}
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* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
  
 
* '''Regulation:''' repression by cysteine ([[T-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]
 
* '''Regulation:''' repression by cysteine ([[T-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7510287 PubMed]

Revision as of 11:10, 12 April 2012

  • Description: cysteine-tRNA synthetase

Gene name cysS
Synonyms snpA
Essential yes PubMed
Product cysteine-tRNA synthetase
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Cys, Met & Sulfate assimilation
MW, pI 53 kDa, 5.07
Gene length, protein length 1398 bp, 466 aa
Immediate neighbours cysE, mrnC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CysS context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU00940

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-270 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1U0B (from Escherichia coli, 42% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References