Difference between revisions of "LutB"
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** induction by lactate [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed] | ** induction by lactate [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed] | ||
** less expressed under conditions of extreme iron limitation ([[FsrA]]) [http://www.ncbi.nlm.nih.gov/pubmed/18697947 PubMed] | ** less expressed under conditions of extreme iron limitation ([[FsrA]]) [http://www.ncbi.nlm.nih.gov/pubmed/18697947 PubMed] | ||
− | ** part of the iron sparing response ([[FsrA]]) {{PubMed| | + | ** part of the iron sparing response, down-regulation in a'' [[fur]]'' mutant ([[Fur]], [[FsrA]], [[FpbB]]) {{PubMed|22427629}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed] | ** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed] | ||
** dual repression by [[LutR]] (inducer lactate) and [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed] | ** dual repression by [[LutR]] (inducer lactate) and [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed] | ||
− | ** [[FsrA]]: translational repression {{PubMed|18697947}} | + | ** [[FsrA]]: translational repression {{PubMed|22427629,18697947}} |
+ | ** [[FbpB]]: acts as RNA chaperone, increases interaction between ''[[fsrA]]'' and ''[[lutA]]-[[lutB]]-[[lutC]]'' RNAs {{PubMed|22427629}} | ||
* '''Additional information:''' | * '''Additional information:''' |
Revision as of 15:26, 25 March 2012
- Description: lactate catabolic enzyme
Gene name | lutB |
Synonyms | yvfW |
Essential | no |
Product | lactate oxidase |
Function | lactate utilization |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 53 kDa, 5.848 |
Gene length, protein length | 1437 bp, 479 aa |
Immediate neighbours | lutC, lutA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
FsrA regulon, LutR regulon, SinR regulon
The gene
Basic information
- Locus tag: BSU34040
Phenotypes of a mutant
no growth with lactate as the single carbon source PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: oxidation of lactate to pyruvate PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): contains an iron-sulfur cluster
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O07021
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
Your additional remarks
References
Gregory T Smaldone, Haike Antelmann, Ahmed Gaballa, John D Helmann
The FsrA sRNA and FbpB protein mediate the iron-dependent induction of the Bacillus subtilis lutABC iron-sulfur-containing oxidases.
J Bacteriol: 2012, 194(10);2586-93
[PubMed:22427629]
[WorldCat.org]
[DOI]
(I p)
Gregory T Smaldone, Olga Revelles, Ahmed Gaballa, Uwe Sauer, Haike Antelmann, John D Helmann
A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.
J Bacteriol: 2012, 194(10);2594-605
[PubMed:22389480]
[WorldCat.org]
[DOI]
(I p)
Yunrong Chai, Roberto Kolter, Richard Losick
A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation.
J Bacteriol: 2009, 191(8);2423-30
[PubMed:19201793]
[WorldCat.org]
[DOI]
(I p)
Ahmed Gaballa, Haike Antelmann, Claudio Aguilar, Sukhjit K Khakh, Kyung-Bok Song, Gregory T Smaldone, John D Helmann
The Bacillus subtilis iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins.
Proc Natl Acad Sci U S A: 2008, 105(33);11927-32
[PubMed:18697947]
[WorldCat.org]
[DOI]
(I p)
Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695]
[WorldCat.org]
[DOI]
(P p)