BshA

• Description: L-malic acid glycosyltransferase, involved in bacillithiol synthesis
  
  

Gene name	bshA
Synonyms	jojH, ypjH
Essential	no
Product	L-malic acid glycosyltransferase
Function	biosynthesis of bacillithiol
MW, pI	41 kDa, 6.149
Gene length, protein length	1131 bp, 377 aa
Immediate neighbours	cca, bshB1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU22460

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed

• Protein family: NamA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3MBO (from B. anthracis) PubMed

• UniProt: P42982

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: bshA::mls available in John_Helmann lab
  • GP88 (ypjH::pX2(cat)), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional references: PubMed