IspF

• Description: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
  
  

Gene name	ispF
Synonyms	yacN
Essential	yes PubMed
Product	2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
Function	isoprenoid biosynthesis
MW, pI	16 kDa, 5.398
Gene length, protein length	474 bp, 158 aa
Immediate neighbours	ispD, gltX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of lipids, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU00910

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)

• Protein family: ispF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q06756

• KEGG entry: [3]

• E.C. number: 4.6.1.12

Expression and regulation

• Operon: yacL-ispD-ispF PubMed

• Sigma factor: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: