Difference between revisions of "MiaA"
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* '''Locus tag:''' BSU17330 | * '''Locus tag:''' BSU17330 | ||
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| + | [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=miaA_1866389_1867333_1 Expression] | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
Revision as of 10:04, 25 January 2012
- Description: tRNA isopentenylpyrophosphate transferase
| Gene name | miaA |
| Synonyms | |
| Essential | no |
| Product | tRNA isopentenylpyrophosphate transferase |
| Function | tRNA modification |
| MW, pI | 35 kDa, 6.002 |
| Gene length, protein length | 942 bp, 314 aa |
| Immediate neighbours | ymaF, hfq |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17330
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)
- Protein family: IPP transferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31795
- KEGG entry: [2]
- E.C. number: 2.5.1.8
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766]
[WorldCat.org]
[DOI]
(I p)
The corresponding gene/ protein in other bacteria
