RapA

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay
  
  

Gene name	rapA
Synonyms	gsiAA, spo0L
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation
Interactions involving this protein in SubtInteract: RapA
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	44 kDa, 4.848
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	yjoB, phrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, quorum sensing

This gene is a member of the following regulons

CodY regulon, ComA regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU12430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • modular organization comprising an amino terminal alpha-helical domain connected to a domain formed by six tetratricopeptide (TPR) repeats PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapA (central TPR3-5 repeats) -PhrA PubMed
  • RapA (N-terminal 60 amino acids) -Spo0F PubMed

• Localization:

Database entries

• Structure:

• UniProt: Q00828

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapA-phrA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (4.2-fold) (CcpA) PubMed
  • repressed by casamino acids PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CcpA: transcription repression PubMed
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed