MtlD

• Description: mannitol-1-phosphate 5-dehydrogenase
  
  

Gene name	mtlD
Synonyms	mtlB
Essential	no
Product	mannitol-1-phosphate 5-dehydrogenase
Function	mannitol utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	40 kDa, 6.291
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	mtlF, ycsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

MtlR regulon

The gene

Basic information

• Locus tag: BSU03990

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-mannitol 1-phosphate + NAD⁺ = D-fructose 6-phosphate + NADH (according to Swiss-Prot)

• Protein family: mannitol dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3H2Z (from Shigella flexneri 2a str. 2457t mutant, 42% identity, 59% similarity)

• UniProt: P42957

• KEGG entry: [3]

• E.C. number: 1.1.1.17

Expression and regulation

• Operon: mtlA-mtlF-mtlD PubMed

• Sigma factor:

• Regulation:
  • induced by mannitol (MtlR) PubMed
  • repressed by glucose (MtlR) PubMed

• Regulatory mechanism:
  • MtlR: transcription activation PubMed

• Additional information:
  • An antisense RNA is predicted for mtlD PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: