Map

• Description: methionine aminopeptidase
  
  

Gene name	map
Synonyms	mapA
Essential	yes PubMed
Product	methionine aminopeptidase
Function	removal of N-terminal methionine from nascent proteins
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Protein secretion
MW, pI	27 kDa, 6.393
Gene length, protein length	744 bp, 248 aa
Immediate neighbours	adk, infA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, protein modification, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01380

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides (according to Swiss-Prot)

• Protein family: peptidase M24A family (according to Swiss-Prot)

• Paralogous protein(s): YflG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2MAT (from Escherichia coli, 46% identity, 65% similarity) PubMed

• UniProt: P19994

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information: strongly expressed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

CongHui You, HongYan Lu, Agnieszka Sekowska, Gang Fang, YiPing Wang, Anne-Marie Gilles, Antoine Danchin
The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis.
BMC Microbiol: 2005, 5;57
[PubMed:16207374] [WorldCat.org] [DOI] (I e)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)