Difference between revisions of "FlhF"
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| − | * '''Description:''' | + | * '''Description:''' signal recognition particle-like GTPase, placement and assembly of flagella <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || signal recognition particle-GTPase | + | |style="background:#ABCDEF;" align="center"| '''Product''' || signal recognition particle-like GTPase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || placement and assembly of polar flagella | |style="background:#ABCDEF;" align="center"|'''Function''' || placement and assembly of polar flagella | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/FlhF FlhF] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 8.719 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 8.719 | ||
| Line 55: | Line 57: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 64: | Line 64: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| + | ** GTPase activity {{PubMed|22056770}} | ||
| + | ** placement of the first flagellar protein, [[FliF]] {{PubMed|22056770}} | ||
| + | |||
* '''Protein family:''' GTP-binding SRP family (according to Swiss-Prot) | * '''Protein family:''' GTP-binding SRP family (according to Swiss-Prot) | ||
| Line 80: | Line 83: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| + | ** interaction with [[YlxH]] activates the GTPase activity of [[FlhF]] {{PubMed|22056770}} | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[YlxH]]-[[FlhF]] ([[YlxH]] binds the GTP-bound homodimer of [[FlhF]]) {{PubMed|22056770}} | ||
* '''[[Localization]]:''' cell membrane (according to Swiss-Prot) | * '''[[Localization]]:''' cell membrane (according to Swiss-Prot) | ||
| Line 87: | Line 92: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PX3 2PX3] (complexed with GTP-Mg2+) | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=2PX3 2PX3] (complexed with GTP-Mg2+) {{PubMed|17699634}} | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=3SYN 3SYN] (complex with its activator [[YlxH]]) {{PubMed|22056770}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q01960 Q01960] | * '''UniProt:''' [http://www.uniprot.org/uniprot/Q01960 Q01960] | ||
| Line 136: | Line 143: | ||
=References= | =References= | ||
| − | <pubmed>1447978,17699634,17850253,15317803,14651647, 9657996,8157612,15175317</pubmed> | + | <pubmed>1447978,17699634,17850253,15317803,14651647, 9657996,8157612,15175317 22056770 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:56, 8 November 2011
- Description: signal recognition particle-like GTPase, placement and assembly of flagella
| Gene name | flhF |
| Synonyms | |
| Essential | no |
| Product | signal recognition particle-like GTPase |
| Function | placement and assembly of polar flagella |
| Interactions involving this protein in SubtInteract: FlhF | |
| MW, pI | 40 kDa, 8.719 |
| Gene length, protein length | 1098 bp, 366 aa |
| Immediate neighbours | flhA, ylxH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
CodY regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16400
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: GTP-binding SRP family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- UniProt: Q01960
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Gert Bange, Nico Kümmerer, Przemyslaw Grudnik, Robert Lindner, Georg Petzold, Dieter Kressler, Ed Hurt, Klemens Wild, Irmgard Sinning
Structural basis for the molecular evolution of SRP-GTPase activation by protein.
Nat Struct Mol Biol: 2011, 18(12);1376-80
[PubMed:22056770]
[WorldCat.org]
[DOI]
(I e)
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
Gert Bange, Georg Petzold, Klemens Wild, Richard O Parlitz, Irmgard Sinning
The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP.
Proc Natl Acad Sci U S A: 2007, 104(34);13621-5
[PubMed:17699634]
[WorldCat.org]
[DOI]
(P p)
Geeske Zanen, Haike Antelmann, Helga Westers, Michael Hecker, Jan Maarten van Dijl, Wim J Quax
FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion.
J Bacteriol: 2004, 186(17);5956-60
[PubMed:15317803]
[WorldCat.org]
[DOI]
(P p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
P B Carpenter, D W Hanlon, G W Ordal
flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP-binding protein.
Mol Microbiol: 1992, 6(18);2705-13
[PubMed:1447978]
[WorldCat.org]
[DOI]
(P p)
