Difference between revisions of "YqiD"
(→Extended information on the protein) |
|||
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' geranyltransferase<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || geranyltransferase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of peptidoglycan and teichoic acid | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of peptidoglycan and teichoic acid | ||
| Line 20: | Line 20: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dxs]]'', ''[[yqiC]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dxs]]'', ''[[yqiC]]'' | ||
|- | |- | ||
| − | |style="background:#FAF8CC;" align="center"|'''[http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14359]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
| − | |||
| − | |||
| − | |||
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yqiD_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yqiD_context.gif]] | ||
| Line 48: | Line 45: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
The mutation of ''yqiD'' predisposes cells to grow without a wall {{PubMed|19212404}} | The mutation of ''yqiD'' predisposes cells to grow without a wall {{PubMed|19212404}} | ||
| + | |||
=== Database entries === | === Database entries === | ||
| Line 57: | Line 55: | ||
Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In ''B. subtilis'' there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane. | Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In ''B. subtilis'' there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane. | ||
| − | |||
Revision as of 08:30, 16 October 2011
- Description: geranyltransferase
| Gene name | yqiD |
| Synonyms | ispA |
| Essential | no |
| Product | geranyltransferase |
| Function | biosynthesis of peptidoglycan and teichoic acid |
| MW, pI | 29 kDa, 5.021 |
| Gene length, protein length | 816 bp, 272 aa |
| Immediate neighbours | dxs, yqiC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24280
Phenotypes of a mutant
The mutation of yqiD predisposes cells to grow without a wall PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In B. subtilis there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane.
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: dimethylallyl pyrophosphate + 2 isopentanyl pyrophosphate -> farnasyl pyrophosphate
- Protein family:
- Paralogous protein(s): HepT
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: None
- Cofactor(s): Magnesium
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P54383
- KEGG entry: [2]
- E.C. number: 2.5.1.1
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
All information about this gene is based on its sequence similarity to the IspA protein of E. coli. There is no published data on the activity of the B. subtilis protein.
References
M Leaver, P Domínguez-Cuevas, J M Coxhead, R A Daniel, J Errington
Life without a wall or division machine in Bacillus subtilis.
Nature: 2009, 457(7231);849-53
[PubMed:19212404]
[WorldCat.org]
[DOI]
(I p)
Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254]
[WorldCat.org]
[DOI]
(P p)
David J Hosfield, Yanming Zhang, Douglas R Dougan, Alexei Broun, Leslie W Tari, Ronald V Swanson, John Finn
Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis.
J Biol Chem: 2004, 279(10);8526-9
[PubMed:14672944]
[WorldCat.org]
[DOI]
(P p)
