Difference between revisions of "CsrA"

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(Extended information on the protein)
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 +
** [[CsrA]] binds the ''[[hag]]'' mRNA to inhibit translation {{PubMed|21895793}}
  
 
* '''Protein family:''' csrA family (according to Swiss-Prot)
 
* '''Protein family:''' csrA family (according to Swiss-Prot)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** [[CsrA]] is sequestered by interaction with [[FliW]] (the two proteins form a conserved module in many bacteria) {{PubMed|21895793}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 
** [[CsrA]]-[[RsmY]] (hypothetical)
 
** [[CsrA]]-[[RsmY]] (hypothetical)
 +
** [[FliW]]-[[CsrA]] {{PubMed|21895793}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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=== Additional information===
 
=== Additional information===
 +
* [[CsrA]] and [[FliW]] form a conserved module in many bacteria {{PubMed|21895793}}
  
 
=Expression and regulation=
 
=Expression and regulation=
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<pubmed>19385727, </pubmed>
 
<pubmed>19385727, </pubmed>
 
==Original Publications==
 
==Original Publications==
<pubmed>16822857,17555441 8969505, </pubmed>
+
<pubmed>16822857,17555441 8969505, 21895793 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:55, 16 September 2011

  • Description: similar to E. coli carbon storage regulator

Gene name csrA
Synonyms yviG
Essential no
Product unknown
Function unknown
Interactions involving this protein in SubtInteract: CsrA
MW, pI 8 kDa, 6.09
Gene length, protein length 222 bp, 74 aa
Immediate neighbours hag, fliW
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CsrA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

RNA binding regulators

This gene is a member of the following regulons

The CsrA regulon: hag

The gene

Basic information

  • Locus tag: BSU35370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: csrA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • CsrA is sequestered by interaction with FliW (the two proteins form a conserved module in many bacteria) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP469 (spc), available in Stülke lab
  • Expression vector:
    • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab
  • lacZ fusion: pGP461 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications