AnsB

• Description: L-aspartase
  
  

Gene name	ansB
Synonyms
Essential	no
Product	L-aspartase
Function	aspartate degradation
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	52 kDa, 5.69
Gene length, protein length	1425 bp, 475 aa
Immediate neighbours	mleN, ansA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

• Locus tag: BSU23570

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-aspartate = fumarate + NH₃ (according to Swiss-Prot)

• Protein family: Aspartase subfamily (according to Swiss-Prot)

• Paralogous protein(s): CitG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 3R6Q (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed

• UniProt: P26899

• KEGG entry: [3]

• E.C. number: 4.3.1.1

Expression and regulation

• Operon: ansA-ansB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of asparagine (AnsR) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Guntur Fibriansah, Vinod Puthan Veetil, Gerrit J Poelarends, Andy-Mark W H Thunnissen
Structural basis for the catalytic mechanism of aspartate ammonia lyase.
Biochemistry: 2011, 50(27);6053-62
[PubMed:21661762] [WorldCat.org] [DOI] (I p)

Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666] [WorldCat.org] [DOI] (I e)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)

D X Sun, P Setlow
Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
J Bacteriol: 1991, 173(12);3831-45
[PubMed:1711029] [WorldCat.org] [DOI] (P p)