TrpS

• Description: tryptophanyl-tRNA synthetase
  
  

Gene name	trpS
Synonyms
Essential	yes PubMed
Product	tryptophanyl-tRNA synthetase
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	37 kDa, 6.542
Gene length, protein length	990 bp, 330 aa
Immediate neighbours	yjbA, oppA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU11420

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:
  • 3PRH (Val144Pro mutant) PubMed
  • 1BM2 (complex with tryptophan, Geobacillus stearothermophilus)
  • 1MAW (complex with ATP, Geobacillus stearothermophilus)

• UniProt: P21656

• KEGG entry: [3]

• E.C. number: 6.1.1.2

Expression and regulation

• Operon: trpS PubMed

• Sigma factor:

• Regulation:
  • induced by tryptophan limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) PubMed

• Additional information:
  • A ncRNA is predicted between trpS and oppA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alicja K Antonczak, Zuzana Simova, Isaac T Yonemoto, Matthias Bochtler, Anna Piasecka, Honorata Czapinska, Andrea Brancale, Eric M Tippmann
Importance of single molecular determinants in the fidelity of expanded genetic codes.
Proc Natl Acad Sci U S A: 2011, 108(4);1320-5
[PubMed:21224416] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A I Lee, J P Sarsero, C Yanofsky
A temperature-sensitive trpS mutation interferes with trp RNA-binding attenuation protein (TRAP) regulation of trp gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(22);6518-24
[PubMed:8932308] [WorldCat.org] [DOI] (P p)

C Condon, M Grunberg-Manago, H Putzer
Aminoacyl-tRNA synthetase gene regulation in Bacillus subtilis.
Biochimie: 1996, 78(6);381-9
[PubMed:8915527] [WorldCat.org] [DOI] (P p)