Difference between revisions of "Sda"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed] | ** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed] | ||
| − | * '''Additional information:''' Sda is rapidly turned over by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}. | + | * '''Additional information:''' |
| + | ** Sda is rapidly turned over by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}. | ||
| + | ** An [[ncRNA|antisense RNA]] is predicted for ''[[sda]]'' {{PubMed|20525796}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | <pubmed>11207367, 16267290, 19465772, 15023339 16796683 19684115 17932079 17350039 19101565 </pubmed> | + | <pubmed>11207367, 16267290, 19465772, 15023339 16796683 19684115 17932079 17350039 19101565 20525796</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:39, 4 May 2011
- Description: developmental checkpoint protein, inhibits the autokinase activity of KinA and KinB, control of the phosphorelay
| Gene name | sda |
| Synonyms | |
| Essential | no |
| Product | developmental checkpoint protein |
| Function | mediates a developmental checkpoint coupling initiation of sporulation (phosphorylation of Spo0A) to the function of replication initiation proteins |
| Function and regulation of this protein in SubtiPathways: Phosphorelay | |
| MW, pI | 6 kDa, 5.605 |
| Gene length, protein length | 156 bp, 52 aa |
| Immediate neighbours | yloV, yqeF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25690
Phenotypes of a mutant
- sporulates at a higher frequency
- will sporulate in the presence of replication stress
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Sda is a checkpoint protein that is upregulated in response to replication stress PubMed. Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation.
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: Q7WY62
- KEGG entry: [3]
- E.C. number:
Additional information
Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.
Expression and regulation
- Regulatory mechanism:
- Additional information:
- Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.
- An antisense RNA is predicted for sda PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
