Difference between revisions of "YwjH"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP819, available in [[Stülke]] lab | ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP819, available in [[Stülke]] lab | ||
+ | ** GP1411 (''ywjH''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [[SPINE]], available in [[Stülke]] lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' |
Revision as of 14:32, 22 February 2011
- Description: transaldolase
Gene name | ywjH |
Synonyms | |
Essential | no |
Product | transaldolase |
Function | pentose phosphate pathway |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 22 kDa, 5.876 |
Gene length, protein length | 636 bp, 212 aa |
Immediate neighbours | murAB, fbaA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37110
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)
- Protein family: Type 3B subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1WX0 (from Thermus thermophilus hb8, 50% identity, 65% similarity)
- UniProt: P19669
- KEGG entry: [3]
- E.C. number: 2.2.1.2
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References