Difference between revisions of "GndA"
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* '''Mutant:''' | * '''Mutant:''' | ||
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| + | ** pGP1789 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP1389]]) (available in [[Stülke]] lab) | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab |
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 14:25, 22 February 2011
- Description: NADP-dependent phosphogluconate dehydrogenase
| Gene name | gndA |
| Synonyms | yqjI |
| Essential | no |
| Product | NADP-dependent phosphogluconate dehydrogenase |
| Function | pentose phosphate pathway |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 51 kDa, 5.076 |
| Gene length, protein length | 1407 bp, 469 aa |
| Immediate neighbours | zwf, polY1 |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23860
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH (according to Swiss-Prot)
- Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible reaction PubMed
- Domains:
- Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: P80859
- KEGG entry: [3]
- E.C. number: 1.1.1.44
Additional information
It contains a cysteine on the active site. The enzyme is a dimer. PubMed
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
