Difference between revisions of "YvcK"
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| − | * '''Description:''' essential for growth under gluconeogenetic conditions <br/><br/> | + | * '''Description:''' essential for growth under gluconeogenetic conditions, required for correct localization of [[ponA|PBP1]] <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown | |style="background:#ABCDEF;" align="center"| '''Product''' || unknown | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || localization of [[ponA|PBP1]] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408 | ||
| Line 32: | Line 32: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[carbon core metabolism]]}} | + | {{SubtiWiki category|[[carbon core metabolism]]}}, {{SubtiWiki category|[[cell shape]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 45: | Line 45: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
** unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}} | ** unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}} | ||
| − | ** filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}} | + | ** filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}}, this is supressed by overexpression of [[MreB]] or by deletion of ''[[ponA]]'' ([[ponA|PBP1]] ) {{PubMed|21320184}} |
=== Database entries === | === Database entries === | ||
| Line 81: | Line 81: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' | + | * '''Localization:''' localized as a helical-like pattern {{PubMed|21320184}} |
=== Database entries === | === Database entries === | ||
| Line 131: | Line 131: | ||
=References= | =References= | ||
| − | <pubmed>9237995 16272399, </pubmed> | + | <pubmed>9237995 16272399, 21320184 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:16, 16 February 2011
- Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
| Gene name | yvcK |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | localization of PBP1 |
| MW, pI | 34 kDa, 5.408 |
| Gene length, protein length | 951 bp, 317 aa |
| Immediate neighbours | yvcL, yvcJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
carbon core metabolism, cell shape
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34760
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0052 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: localized as a helical-like pattern PubMed
Database entries
- Structure:
- UniProt: O06974
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Boris Görke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
Your additional remarks
References
