Difference between revisions of "SubtInteract"

From SubtiWiki
Jump to: navigation, search
(Created page with "Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as ...")
 
Line 2: Line 2:
  
 
==Methods to detect protein-protein interactions==
 
==Methods to detect protein-protein interactions==
*
+
* Bacterial Two Hybrid System: [[BACTH]]
 +
 
 +
* Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}}
 +
 
 +
* [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking
 +
 
 +
* TAP-Tag purification {{PubMed|10504710,11403571}}
 +
 
 +
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}}
 +
 
 +
==Important publications==
 +
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303  </pubmed>

Revision as of 21:05, 3 January 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Bacterial Two Hybrid System: BACTH
  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Important publications