MetK

• Description: S-adenosylmethionine synthetase
  
  

Gene name	metK
Synonyms	metE
Essential	yes PubMed
Product	S-adenosylmethionine synthetase
Function	S-adenosylmethionine synthesis
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	43 kDa, 5.025
Gene length, protein length	1200 bp, 400 aa
Immediate neighbours	asnB, pckA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU30550

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-methionine + H₂O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)

• Protein family: AdoMet synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1FUG (from Escherichia coli, 63% identity, 75% similarity) PubMed

• UniProt: P54419

• KEGG entry: [3]

• E.C. number: 2.5.1.6

Expression and regulation

• Operon: metK PubMed

• Sigma factor:

• Regulation:
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism:
  • S-box: riboswitch, binds S-adenosylmethionine resulting in transcription termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP432 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

Brooke A McDaniel, Frank J Grundy, Vineeta P Kurlekar, Jerneja Tomsic, Tina M Henkin
Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.
J Bacteriol: 2006, 188(10);3674-81
[PubMed:16672621] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)