Difference between revisions of "Mdh"
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<br/><br/><br/><br/><br/><br/> | <br/><br/><br/><br/><br/><br/> | ||
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[carbon core metabolism]]}}, | ||
| + | {{SubtiWiki category|[[membrane proteins]]}}, | ||
| + | {{SubtiWiki category|[[phosphoproteins]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[CcpA regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[CcpC regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 22:20, 8 December 2010
- Description: malate dehydrogenase
| Gene name | mdh |
| Synonyms | citH |
| Essential | no |
| Product | malate dehydrogenase |
| Function | TCA cycle |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 33 kDa, 4.727 |
| Gene length, protein length | 936 bp, 312 aa |
| Immediate neighbours | phoP, icd |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
carbon core metabolism, membrane proteins, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = oxaloacetate + NADH (according to Swiss-Prot)
- Protein family: MDH type 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- Modification: phosphorylation on Ser-149 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed
Database entries
- Structure: 1EMD (E.coli)
- UniProt: P49814
- KEGG entry: [3]
- E.C. number: 1.1.1.37
Additional information
The enzyme is a tetramer PubMed
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP719 (spc), available in Stülke lab
- Expression vector:
- pGP1123 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP385, available in Stülke lab
- pGP1755 (expression / purification of Mdh-S149A, with N-terminal His-tag from E. coli, in pWH844), available in Stülke lab
- pGP1764 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Stülke lab)
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
