Difference between revisions of "OpuE"
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[transporters/ other]]}}, | ||
| + | {{SubtiWiki category|[[coping with hyper-osmotic stress]]}}, | ||
| + | {{SubtiWiki category|[[membrane proteins]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
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=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 17:25, 8 December 2010
- Description: proline transporter
| Gene name | opuE |
| Synonyms | yerK |
| Essential | no |
| Product | proline transporter |
| Function | compatible solute transport |
| Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress | |
| MW, pI | 53 kDa, 9.815 |
| Gene length, protein length | 1476 bp, 492 aa |
| Immediate neighbours | sapB, gatC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
transporters/ other, coping with hyper-osmotic stress, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06660
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: View classification (according to Swiss-Prot)
- Paralogous protein(s): YcgO
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O06493
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany homepage
Your additional remarks
References
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
F Spiegelhalter, E Bremer
Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A- and sigma B-dependent stress-responsive promoters.
Mol Microbiol: 1998, 29(1);285-96
[PubMed:9701821]
[WorldCat.org]
[DOI]
(P p)
C von Blohn, B Kempf, R M Kappes, E Bremer
Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.
Mol Microbiol: 1997, 25(1);175-87
[PubMed:11902719]
[WorldCat.org]
[DOI]
(P p)
