Difference between revisions of "Easter egg"

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Line 1: Line 1:
−
* '''Description:''' RNA component of [[RNase]] P <br/><br/>
+
* '''Description:''' 6S RNA<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''rnpB''
+
|''bsrB''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rnaP ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA component of ribonuclease P <br/>([[RNase]] P) (catalytic subunit, ribozyme)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || 6S RNA
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || cleavage of precursor sequences <br/>from the 5' ends of pre-tRNAs
+
|style="background:#ABCDEF;" align="center"|'''Function''' || antagonist of [[SigA]]
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length''' ||  401 bp
+
|style="background:#ABCDEF;" align="center"| '''Gene length''' ||  203 bp
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ypsC]]'', ''[[ypsB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yocI]]'', ''[[azoR1]]''
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/rnpB_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt    Gene sequence      (+200bp)  ]'''  
−
 
 
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:rnpB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:BsrB_context.gif]]
−
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
 
|-
 
|-
 
|}
 
|}
Line 28: Line 26:
 
__TOC__
 
__TOC__
  
−
<br/><br/><br/><br/><br/><br/><br/>
+
<br/><br/>
  
 +
<br/><br/><br/><br/><br/>
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
−
{{SubtiWiki category|[[Rnases]]}},
+
{{SubtiWiki category|[[sigma factors and their control]]}},
−
{{SubtiWiki category|[[translation]]}},
 
 
{{SubtiWiki category|[[ncRNA]]}}
 
{{SubtiWiki category|[[ncRNA]]}}
  
Line 48: Line 46:
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bsrB.html]
−
 
 
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG00045]
 
−
 
 
−
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2A64 2A64] (from Geobacillus stearothermophilus),  [http://www.rcsb.org/pdb/explore.do?structureId=1NBS 1NBS] (specificity domain)
 
  
 +
* '''SubtiList entry:''' no entry
 +
 +
* '''Structure:'''
  
 +
=== Additional information===
  
−
=The RNA=
 
  
−
* '''Biological activity:''' 5' end maturation of precursor tRNAs
 
  
−
* '''RNA family:'''
 
−
 
−
* '''cofactor:''' divalent cations (Ca(II) or Mg (II)) for the stabilization of the complex with pre-tRNA {{PubMed|20434461}}
 
−
 
−
* '''interaction:''' [[RnpA]]-RnpB
 
−
 
−
=== Additional information===
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''bsrB'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11886746 PubMed]
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
  
−
* '''Regulation:'''  
+
* '''Regulation:''' expressed during vegetative growth[http://www.ncbi.nlm.nih.gov/sites/entrez/11886746 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 88: Line 77:
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
−
* '''GFP fusion:'''
 
  
−
* '''two-hybrid system:'''
+
=Labs working on this gene/RNA=
  
−
* '''Antibody:'''
+
* Kouji Nakamura, Tsukuba, University, Japan
−
 
+
* [[Roland Hartmann]], Philipps-Universität Marburg, Marburg, Germany, [http://www.uni-marburg.de/fb16/ipc/ag_hartmann homepage]
−
=Labs working on this gene/RNA=
 
−
* [[Carol Fierke]], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
 
−
* [[Roland Hartmann]], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=
  
 
=References=
 
=References=
−
==Reviews==
 
−
<pubmed> 19215776 17868095 14691942 16650980 16980936 16679018 14550630 </pubmed>
 
  
−
==Original Publications==
+
<pubmed>15811922,,11886746 15811922, </pubmed>
−
<pubmed>16470227,17355991,11812129,10390342,11454206, 19549719,11454206, 19717440 ,20133747  19932118 20188108 20434461 17299131 14510496 12951523 12836338 11577704 20557101 </pubmed>
 
  
 
[[Category:small RNA]]
 
[[Category:small RNA]]

Revision as of 13:50, 8 December 2010

  • Description: 6S RNA

Gene name bsrB
Synonyms
Essential no
Product 6S RNA
Function antagonist of SigA
Gene length 203 bp
Immediate neighbours yocI, azoR1
Gene sequence (+200bp)
Genetic context
BsrB context.gif








Categories containing this gene/protein

sigma factors and their control, ncRNA

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: no entry
  • Structure:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: expressed during vegetative growthPubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:


Labs working on this gene/RNA

  • Kouji Nakamura, Tsukuba, University, Japan
  • Roland Hartmann, Philipps-Universität Marburg, Marburg, Germany, homepage

Your additional remarks

References

Jeffrey E Barrick, Narasimhan Sudarsan, Zasha Weinberg, Walter L Ruzzo, Ronald R Breaker
6S RNA is a widespread regulator of eubacterial RNA polymerase that resembles an open promoter.
RNA: 2005, 11(5);774-84
[PubMed:15811922] [WorldCat.org] [DOI] (P p)

Yoshinari Ando, Sayaka Asari, Satoru Suzuma, Kunio Yamane, Kouji Nakamura
Expression of a small RNA, BS203 RNA, from the yocI-yocJ intergenic region of Bacillus subtilis genome.
FEMS Microbiol Lett: 2002, 207(1);29-33
[PubMed:11886746] [WorldCat.org] [DOI] (P p)

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