FadB

• Description: 3-hydroxyacyl-CoA dehydratase
  
  

Gene name	fadB
Synonyms	ysiB
Essential	no
Product	3-hydroxyacyl-CoA dehydratase
Function	fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	27 kDa, 4.951
Gene length, protein length	774 bp, 258 aa
Immediate neighbours	etfB, fadR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of lipids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H₂O (according to Swiss-Prot)

• Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3PEA (from B. anthracis, 49% identity, 66% similarity)

• UniProt: P94549

• KEGG entry: [3]

• E.C. number: 4.2.1.17

Expression and regulation

• Operon:
  • lcfA-fadR-fadB-etfB-etfA PubMed
  • fadR-fadB-etfB-etfA PubMed

• Sigma factor:
  • fadR: SigA PubMed

• Regulation:
  • induced by long-chain fatty acids (FadR) PubMed
  • repressed by glucose (3.8-fold) (CcpA) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)