Difference between revisions of "CheB"
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= Categories containing this gene/protein = | = Categories containing this gene/protein = | ||
| − | {{SubtiWiki category|[[motility and chemotaxis]]}} | + | {{SubtiWiki category|[[motility and chemotaxis]]}}, {{SubtiWiki category|[[phosphoproteins]]}} |
=The protein= | =The protein= | ||
| Line 69: | Line 69: | ||
* '''Domains:''' | * '''Domains:''' | ||
| − | * '''Modification:''' | + | * '''Modification:''' phosphorylated on a Asp residue by [[CheA]] {{PubMed|11553614}} |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| Line 132: | Line 132: | ||
<pubmed> 20122866 </pubmed> | <pubmed> 20122866 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>10632876,12123464,1905718,14731274,17850253,14651647, 9657996,8157612,15175317</pubmed> | + | <pubmed>10632876,12123464,1905718,14731274,17850253,14651647, 9657996,8157612,15175317 11553614</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:05, 1 December 2010
- Description: MCP-glutamate methylesterase / two-component response regulator-like
| Gene name | cheB |
| Synonyms | cheL |
| Essential | no |
| Product | MCP-glutamate methylesterase / two-component response regulator-like |
| Function | motility, chemotaxis |
| MW, pI | 38 kDa, 8.119 |
| Gene length, protein length | 1071 bp, 357 aa |
| Immediate neighbours | ylxH, cheA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU16420
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
Categories containing this gene/protein
motility and chemotaxis, phosphoproteins
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q05522
- KEGG entry: [3]
- E.C. number: 3.1.1.61
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866]
[WorldCat.org]
[DOI]
(I p)
Original publications
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Michael W Bunn, George W Ordal
Receptor conformational changes enhance methylesterase activity during chemotaxis by Bacillus subtilis.
Mol Microbiol: 2004, 51(3);721-8
[PubMed:14731274]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
Michael A Zimmer, Hendrik Szurmant, Michael M Saulmon, Marissa A Collins, Jason S Bant, George W Ordal
The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Mol Microbiol: 2002, 45(2);555-68
[PubMed:12123464]
[WorldCat.org]
[DOI]
(P p)
E Karatan, M M Saulmon, M W Bunn, G W Ordal
Phosphorylation of the response regulator CheV is required for adaptation to attractants during Bacillus subtilis chemotaxis.
J Biol Chem: 2001, 276(47);43618-26
[PubMed:11553614]
[WorldCat.org]
[DOI]
(P p)
J R Kirby, T B Niewold, S Maloy, G W Ordal
CheB is required for behavioural responses to negative stimuli during chemotaxis in Bacillus subtilis.
Mol Microbiol: 2000, 35(1);44-57
[PubMed:10632876]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
D S Bischoff, G W Ordal
Sequence and characterization of Bacillus subtilis CheB, a homolog of Escherichia coli CheY, and its role in a different mechanism of chemotaxis.
J Biol Chem: 1991, 266(19);12301-5
[PubMed:1905718]
[WorldCat.org]
(P p)
