RpsD

• Description: ribosomal protein
  
  

Gene name	rpsD
Synonyms
Essential	yes PubMed
Product	ribosomal protein S4 (BS4)
Function	translation
MW, pI	22 kDa, 10.243
Gene length, protein length	600 bp, 200 aa
Immediate neighbours	ytrP, tyrS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29660

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S4P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1C06 (Geobacillus stearothermophilus)

• UniProt: P21466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rpsD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

T Inaoka, K Kasai, K Ochi
Construction of an in vivo nonsense readthrough assay system and functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus subtilis.
J Bacteriol: 2001, 183(17);4958-63
[PubMed:11489846] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
Characterization of the Bacillus subtilis rpsD regulatory target site.
J Bacteriol: 1992, 174(21);6763-70
[PubMed:1400226] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The rpsD gene, encoding ribosomal protein S4, is autogenously regulated in Bacillus subtilis.
J Bacteriol: 1991, 173(15);4595-602
[PubMed:1906866] [WorldCat.org] [DOI] (P p)

T M Henkin, G H Chambliss, F J Grundy
Bacillus subtilis mutants with alterations in ribosomal protein S4.
J Bacteriol: 1990, 172(11);6380-5
[PubMed:2121712] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4.
J Bacteriol: 1990, 172(11);6372-9
[PubMed:1699930] [WorldCat.org] [DOI] (P p)

T M Henkin, G H Chambliss
Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4.
Mol Gen Genet: 1984, 193(2);364-9
[PubMed:6420647] [WorldCat.org] [DOI] (P p)